The invention discloses a multi-stage flow channel
blood vessel chip based on additive manufacturing in the field of bionic chips, the multi-stage flow channel
blood vessel chip comprises a substrate and a double-layer
blood vessel channel located on the surface of the substrate, the double-layer blood vessel channel comprises an inlet and an outlet which are used for circularly introducing intravenous endothelial cells, and a closure contraction part is arranged between the inlet and the outlet; the closure contraction part comprises elastic salient points which define a rectangular matrix, and the
radius of the elastic salient points is larger than the distance between the elastic salient points. According to the technical scheme, a blood vessel
chip structure is designed according to the characteristics of
skull capillaries, so that in-vitro culture of the
stent and the HUVECs is implemented, a blood vessel forming mechanism is analyzed, the cost of in-vitro culture of organs is reduced, an innovative design concept of the blood vessel embedded
stent with the balustrade
vein characteristics is provided, and the
stent has a good application prospect. And in combination with in-vitro culture of dynamic
perfusion, the vascularization process is disclosed better, and a new way is provided for regeneration of bone tissues.