This utility model discloses a positioning fixture, comprising: a base; multiple sliding members disposed on the base and movable along a first direction; a positioning member mounted on the sliding members and movable along a second direction; and a locking member mounted on the sliding members, including a force-applying part and a fastening part. The force-applying part can move along a preset path, and when it moves along the preset path, it drives the fastening part to abut against the workpiece, thereby fixing the workpiece in contact with the positioning member. The positioning fixture of this utility model allows adjustment of the positions of the sliding members and the positioning member according to the shape of the workpiece. The force-applying part of the locking member then drives the fastening part to abut against the workpiece, thus clamping and fixing the workpiece through the positioning member and the fastening part. The positions of the sliding members, positioning member, and locking member of this utility model are all flexibly adjustable, thus enabling the fixing of irregularly shaped workpieces.