The invention provides a physiological sample storage device with a high multiple-protection effect. The physiological sample storage device comprises a storage box, a sealing door, an observation window, a door handle, a supporting edge, moving wheels, a brake pad, a cold fan, a gas conveying pipeline, an activated carbon filter screen, a temperature and humidity sensor, a PLC, a display screen,switches, an assembling plate, a fluorescent lamp, an ultraviolet lamp, an atomizer, a rotary observation frame structure, a reinforcing protection frame structure, an adjustment movable seat structure and a protection side plate structure; the sealing door is mounted on the right side of the front surface of the storage box through hinges; the observation window is inlaid in the middle of the interior of the sealing door; and the door handle is welded to the middle part of the right side of the rear wall of the sealing door. The physiological sample storage device has the advantages that dueto the arrangement of the rotary observation frame structure, when a sample utensil is placed, the sample utensil can be placed close to a corresponding placing plate, and meanwhile, the sample utensil is in an inclined shape, so that a worker can conveniently check the condition of the sample utensil.