The invention discloses an air conditioner aggregation control method and system and a regulation and control device, and belongs to the field of power system demand response load aggregation control. According to a load aggregation control target and based on the historical feedback of a user in response to a regulation instruction, the high probability estimation of receiving a regulation instruction, the small response behavior variance and the small number of historical times nt,i of sent instruction in historical feedback are adopted as reference, the sorting index values vt,i of users are set, and m users are sequentially selected to send adjusting instructions from large to small according to the sorting index values vt,i until satisfying the formula shown as the specification, and if a formula shown as the specification is satisfied, m is equal to n, P1,..., Pn represent the air conditioner power of the users respectively, and n is the total number of the users. According to the invention, a more ideal secondary frequency modulation effect is realized, the blind sending of adjustment instructions to users is avoided, and the number of users sending instructions and the number of users selecting to quit the response are reduced, so that the cost of a load aggregator and the fatigue of the users due to frequent receiving of the adjustment instructions are reduced.