The invention discloses a multi GPU calculation based reverse time migration imaging method of a 3D TTI medium. According to the method, a stable second-order coupling equation set serves as a calculation model, finite SV wave components are given to enhance the wave field spreading stability, an anisotropy parameter matching method is used to suppress interference of pseudo-transverse waves, uniform anisotropy boundaries corresponding to a random speed boundary and other parameters are introduced, storage of large amount of wave field data and frequency disk reading and writing are avoided, and a GPU-based CUDA platform is used to realize high-performance parallel calculation. According to the invention, a second-order coupling quasi-acoustic equipment in the TTI medium is solved in a difference method on the basis of multi-GPU parallel calculation technology, wave field spreading is stable, pseudo-transverse waves are suppressed effectively, application of the random boundary reduces wave field storage and disk reading and writing, a multi-asynchronous-flow high parallel strategy of multi GPU calculation is used, the calculation efficiency is improved obviously, and the imaging method meets requirements for industrialization of anisotropy reverse time migration.