59 results about "Pentatricopeptide repeat" patented technology

The invention belongs to the technical field of toughened and modified pentatricopeptide repeats (PPR), and discloses a toughened and modified PPR extrusion pipe material and a preparation method thereof. The toughened and modified PPR extrusion pipe material comprises the following components by mass percent: 58-98% of PPR, 1-20% of a toughening agent, 1-20% of poly 1-butene, 0-1% of a heat stabilizer and 0-1% of a nucleating agent special for polypropylene. According to the method, synergistic toughening of the traditional toughening agent and novel toughening agent of poly 1-butene is adopted, so that the low-temperature toughness of the PPR extrusion pipe material is improved, and the welding strength and heat resistance are ensured. The tensile strength at 23 DEG C is greater than or equal to 25MPa, the elongation at break at 23 DEG C is greater than or equal to 500%, the tensile strength at 95 DEG C is greater than or equal to 10MPa, the low-temperature notch impact strength at 30 DEG C below zero is greater than or equal to 5KJ / m<2>, and the vicat softening temperature (10N) is greater than or equal to 125 DEG C.

The invention discloses a novel high-efficiency PPR (pentatricopeptide repeat) pipe cold-resistant toughening color master batch. The color master batch comprises the following components in parts by weight: 5-25 parts of polypropylene, 5-10 parts of pigment, 35-65 parts of cold-resistant modifying agent and 1-15 parts of reinforced modifying agent, wherein the cold-resistant modifying agent is one substance or a mixture of more of styrene-butadiene-styrene ternary segmented copolymer, ethylene-vinyl acetate copolymer and ethylene-octylene copolymer, and the reinforced modifying agent is one substance or a mixture of more of nano calcium carbonate, nano silicon and the nano barium sulfate. According to the color master batch, the formula components of the color master batch can be optimized and improved, so that the novel high-efficiency PPR pipe cold-resistant toughening color master batch can be prepared. The color master batch provided by the invention not only can play a role in coloring, but also can obviously improve the flexibility and the shock strength of the PPR pipe under the condition of low temperature, so that the cold resistance of the pipe can be improved, and the service life of the pipe can be prolonged.

The invention discloses a partially-decoupled plane three-freedom-degree parallel precision positioning platform. The partially-decoupled plane three-freedom-degree parallel precision positioning platform comprises a movable platform and a fixed platform, wherein three groups of driving branches with the same structures are arranged between the movable platform and the fixed platform; and every group of driving branch comprises a pentatricopeptide repeats (PPR) driving chain which is formed by sequentially connecting a first movable pair, a second movable pair and a rotary pair. According to the PPR driving chain, a guide rail of the first movable pair is fixedly connected with the fixed platform, a guide rail of the second movable pair is fixedly connected with a slider of the first movable pair, one end of a rotary hinge of the rotary pair is connected with a slider of the second movable pair, and the other end of the rotary hinge of the rotary pair is connected with the movable platform. A motion input part is decoupled, and motion input in the X direction (Y direction) cannot cause driven motion in the X direction (Y direction). By the easy technical means, the defects that the conventional parallel mechanism is complicated in kinematic coupling and kinematic solution and fussy in designing and machining, and the like are overcome, the requirement for high precision of the precision positioning platform can be met, and the partially-decoupled plane three-freedom-degree parallel precision positioning platform has wide application prospect in the fields of precision machining, microelectronic manufacturing and the like.

The object of the present invention is to isolate Rf gene, particularly Rf1 gene derived from radish, and identify its structure. The present invention provides a protein involved in restoration of a cytoplasmic male sterile individual to fertility which has 14 or more pentatricopeptide repeat (hereafter may be abbreviated to PPR) motifs, wherein a group of the motifs is divided into 3 or more blocks, each of the individual blocks has at least 2 or more PPR motifs, and the block in a carboxyl terminal (C terminal) side has 4 PPR motifs.

The invention relates to a nucleic acid aptamer R14 which can specifically recognizing LRPPRC (leucine rich pentatricopeptide repeat containing), and the sequence of the nucleic acid aptamer R14 is shown in SEQ ID NO: 1. The nucleic acid aptamer R14 is capable of inhibiting tumor cell proliferation by blocking the normal tissue function of the LRPPRC and interfering the cell cycle progression. Theinvention further provides application of the nucleic acid aptamer R14 specifically recognizing the LRPPRC and SRC kinase inhibitor in combined treatment of tumour. The nucleic acid aptamer R14 is capable of enhancing the sensitivity of tumour to the SRC kinase inhibitor, and the SRC kinase inhibitor can be used for eliminating SRC kinase activity abnormal activation caused by the nucleic acid aptamer R14.

The invention relates to a framework of a greenhouse shed, which comprises vertical rods perpendicularly fixed on the ground, diagonal rods, transverse rods and connecting parts, wherein the transverse rods are perpendicular to the planes where the vertical rods and the diagonal rods are located, and the connecting parts are connected among the vertical rods, the diagonal rods and the transverse rods. The vertical rods, the diagonal rods and the transverse rods are all made of pentatricopeptide repeat (PPR) materials. The framework made of the PPR materials is hygienic, environment-friendly, energy-saving, resistant to temperature, pressure, cold water and hot water, good in chemical stability, and strong in corrosion resistance. By means of hot-melt connection, the framework is free of leakage, safe and reliable. The service life of the framework is as long as more than 50 years. In addition, the price of the PPR materials is far lower than that of lagging jack materials for making hot galvanizing thin-wall steel tubes so that the framework is practical and economical.

The invention belongs to novel polymer composite materials and relates to a PPR pipe material and a manufacturing method thereof. The material is composed of the following components in parts by weight: 60-84 parts of polypropylene random copolymer, 6-40 parts of flame retardant, 0.75-1.5 parts of antistatic agent and 0.3-0.6 part of antioxidant. The flame retardant is added to improve the flame retardant property of the PPR pipe material, the antistatic agent is added to improve the antistatic property of the PPR pipe material, and the flame-retardant and antistatic PPR pipe material provided by the invention is simple in preparation process and easy to process.

A PPR (pentatricopeptide repeats) pipe casing lamination-injection mould comprises an upper mould cavity and a lower mould cavity composed of a fixed mould cavity plate, a moving mould cavity plate and a moving mould plate. Each of the upper mould cavity and the lower mould cavity contains a plastic part. Large slides are disposed on two sides of the moving mould plate and driven by inclined guide posts. Slider cores are mounted on the inner side of the large sliders and extend into the inner wall of a plastic part. The PPR pipe casing lamination-injection mould is characterized in that runner inserts, a runner cutoff mechanism and a top branch runner device are mounted between the large sliders, the runner cutoff mechanism comprises a fixed cutting block which is connected with a first cut piece and a second cut piece through positioning pins, the first cut piece and second cut piece are inserted into the runner inserts, the top branch runner device comprises connecting blocks, a limit screw is mounted on each connecting block and connected with the large sliders, one connecting block is connected with a corresponding top plate wedge, runner ejector pins, ejector springs and top plates are mounted in the moving mould plate, and the head of each too plate is abutted to a distribution handle and provided with a tapped wedged groove matched with a corresponding tapered wedge.

The invention discloses a water feeding pipeline with a polystyrene heat preservation layer. The water feeding pipeline is formed by a PPR (Pentatricopeptide Repeats) pipe, a polyethylene heat preservation layer and a fiberglass protection shell, wherein the polyethylene heat preservation layer is used outside the PPR pipe, and a heat preservation tile in the position of a connector is coiled and coated by using an aluminum foil tape; the fiberglass protection shell is arranged outside the polyethylene heat preservation layer; an elbow position of the PPR pipe and a pipeline connecting position are buckled by using a polyethylene heat preservation tile and then are well coiled and fixed by the aluminum foil tape; the elbow position of the PPR pipe and the pipeline connecting position are buckled by using a fiberglass protection tile and then are well coiled and fixed by the aluminum foil tape. According to the water feeding pipeline disclosed by the invention, the thickness of the polystyrene heat preservation layer is designed in a way that a severe cold region is divided into three stages according to different frost lines, different recommended embedding depths can be given for different stages, and the thickness of the heat perservation is calculated after the embedding depth is determined, therefore, the water feeding pipeline is more scientific, and the problem of pipeline frost heaving can be effectively solved.

The invention provides an OsPPR34 gene which is separated and cloned from rice DNA and is related to a rice plant type and drought resistance. The protein coded by the gene contains a conservative P structural domain of a PPR (pentatricopeptide repeat) family, and participates in RNA editing of a mitochondrial gene. The OsPPR34 gene regulates and controls the plant height of the rice through a gibberellin way, meanwhile, the root tropism is changed, the deep root ratio of a knockout mutant of the OsPPR34 gene is remarkably increased, and the OsPPR34 gene shows high drought resistance in a root canal experiment. The OsPPR34 gene regulates and controls the plant height and the drought resistance of the rice, the plant height of a knockout mutant of the OsPPR34 gene is reduced, and the OsPPR34 gene has a high depth-to-root ratio and can be applied to improvement of the plant type of the cultivated rice and improvement of the drought resistance of the cultivated rice.

The invention relates to the field of plant genetic engineering, and specifically discloses a cytoplasmic male sterility restorer gene in rice and an application thereof. The invention provides a nucleotide sequence of a wild abortive cytoplasmic male sterility restorer gene (i) Rf4 ( / i) isolated and cloned from rice and an amino acid polypeptide sequence encoded by the restorer gene. The gene belongs to a member of PPR (pentatricopeptide repeats) gene family and is a constitutively expressed gene. The fertility is restored by negatively regulating the expression levels of sterility gene (i) WA352 ( / i) in mitochondrial genome. A new restorer line can be cultivated by transforming (i) Rf4 ( / i) into the original rice varieties containing no restorer gene. Transgenic restorer lines are hybridized with a wild abortive type sterile line to produce hybrids having normal sterility for production.

The object of the present invention is to isolate Rf gene, particularly Rf1 gene derived from radish, and identify its structure. The present invention provides a protein involved in restoration of a cytoplasmic male sterile individual to fertility which has 14 or more pentatricopeptide repeat (hereafter may be abbreviated to PPR) motifs, wherein a group of the motifs is divided into 3 or more blocks, each of the individual blocks has at least 2 or more PPR motifs, and the block in a carboxyl terminal (C terminal) side has 4 PPR motifs.

The invention discloses a water feeding pipeline with a polystyrene heat preservation layer. The water feeding pipeline is formed by a PPR (Pentatricopeptide Repeats) pipe, a polyethylene heat preservation layer and a fiberglass protection shell, wherein the polyethylene heat preservation layer is used outside the PPR pipe, and a heat preservation tile in the position of a connector is coiled and coated by using an aluminum foil tape; the fiberglass protection shell is arranged outside the polyethylene heat preservation layer; an elbow position of the PPR pipe and a pipeline connecting position are buckled by using a polyethylene heat preservation tile and then are well coiled and fixed by the aluminum foil tape; the elbow position of the PPR pipe and the pipeline connecting position are buckled by using a fiberglass protection tile and then are well coiled and fixed by the aluminum foil tape. According to the water feeding pipeline disclosed by the invention, the thickness of the polystyrene heat preservation layer is designed in a way that a severe cold region is divided into three stages according to different frost lines, different recommended embedding depths can be given for different stages, and the thickness of the heat perservation is calculated after the embedding depth is determined, therefore, the water feeding pipeline is more scientific, and the problem of pipeline frost heaving can be effectively solved.

The invention relates to an antibody combination and a kit for quantitatively detecting serum LRPPRC (leucine-rich pentatricopeptide repeat motif protein) to early diagnose cancers. The antibody combination comprises: (a) a polyclonal antibody that specifically binds to the 611th to 907th amino acid fragment of the LRPPRC; and (b) a monoclonal antibody that specifically binds to the 964th to 1273th amino acid fragment of the LRPPRC. The two antibodies for different immunogenic fragments are used to detect the LRPPRC content of serum in order to establish a sensitive and specific early cancer diagnosis method.

The utility model discloses a high-performance fiber reinforced PPR (pentatricopeptide repeats) pipe. The pipe sequentially comprises an inner layer (1), a fiber reinforced layer (2) and an outer layer (3) from inside to outside, and the fiber reinforced layer (2) is formed by winding at least two fiber bundles. The pipe has the characteristics of high toughness and high strength, and overcomes the defects of low temperature resistance, poor shear resistance and high cost caused by manufacturing materials.

The embodiment of the invention discloses an LRPPRC negative regulating agent and application thereof. According to the implementation manner of the LRPPRC negative regulating agent, compared with theprior art, high-expression LRPPRC in tumor cells is taken as a therapeutic target, gossypol acetate is taken as a targeted therapeutic drug, and through specific binding of the therapeutic target andthe targeted therapeutic drug, the aim of inhibiting growth of the tumor cells can be achieved; meanwhile, the gossypol acetate is a clinically applicable compound, so that the LRPPRC negative regulating agent is high in safety, simple in mode of administration, wide in raw material resource, low in price and capable of reducing the treatment costs for the patient.

The utility model relates to the technical field of pipeline connection, and discloses a clamping structure for a PPR pipe, which is characterized in that a first clamping piece is fixedly arranged at one end of a first pipe, a clamping groove is arranged on the first clamping piece, and the clamping groove is provided with an unlocking section and a locking section which are communicated with each other; the second pipe and the first pipe are coaxially arranged, a second clamping piece is movably arranged at the end, opposite to the first clamping piece, of the second pipe, an unlocking position and a locking position are arranged on the movement path of the second clamping piece, when the second clamping piece is located at the unlocking position, the second clamping piece is located in the unlocking section, and when the second clamping piece is located at the locking position, the second clamping piece is located in the locking section. The second clamping piece is located in the locking section; the adjusting ring is rotatably arranged on the second pipe, and the adjusting ring drives the second clamping piece to enter the locking section from the unlocking section and enter the unlocking section from the locking section. According to the clamping structure for the PPR pipe, the first pipe and the second pipe can be quickly connected in a butt joint mode, the disassembly and assembly efficiency is high, and the installation efficiency is improved accordingly.

The invention discloses a photosynthesis regulatory protein LPE1 and an application thereof. A photosynthesis regulatory protein mutant lpe1 is screened from an Arabidopsis thaliana mutant library by inventors, a gene LPE1 (AT3G46610), of which codes contain a chloroplast protein of a PPR (pentatricopeptide repeat) structure field, is cloned through molecular identification, and the condition that the LPE1 participates in the light effect regulation and control of Arabidopsis thaliana is confirmed; two LPE1 interference strain lines and one homozygous deletion mutant strain line are obtained by using a forward genetics method, and the condition that the afunction of the gene LPE1 causes the developmental retardation of plants and the decrease of maximum photosynthetic efficiency is discovered; and more importantly, the protein LPE1 has sequence conservation in other crops, and the condition that functions of the LPE1 in the other crops may also be of conservation is prompted, so that crops with high photosynthetic efficiency are advantageously obtained through regulating and controlling the LPE1 by means of genetic engineering.

The utility model discloses a PPR (pentatricopeptide repeats) lining copper reinforced composite pipe which comprises a PPR pipe body, a lining copper layer is fixedly connected to the outer side of the PPR pipe body, a first reinforcing layer is fixedly connected to the outer side of the lining copper layer, a reinforcing unit is arranged on the outer side of the first reinforcing layer, a second reinforcing layer is arranged on the outer side of the reinforcing unit, and the outer side of the second reinforcing layer is fixedly connected with the outer side of the lining copper layer. The outer side of the second reinforcing layer is fixedly connected with a high-temperature-resistant layer, the outer side of the high-temperature-resistant layer is fixedly connected with a wear-resistant layer, the reinforcing unit comprises a covering layer, the covering layer is fixedly connected between the first reinforcing layer and the second reinforcing layer, and the inner side of the covering layer is fixedly connected with a plurality of reinforcing rings. And through the arrangement of the first reinforcing layer, the second reinforcing layer, the reinforcing rings, the reinforcing rods, the filling rods and the covering layer, the overall compression resistance of the PPR pipe can be conveniently improved, and the situation that during carrying, the PPR pipe is pressed and damaged by external pressure, and liquid conveying is affected is avoided.

The utility model relates to the technical field of extrusion molding, in particular to a molding output device for extrusion molding discharge, which comprises a PPR (pentatricopeptide repeats) pipe extrusion molding device, a cooling bin is arranged on one side of the PPR pipe extrusion molding device, a cut-off bin is arranged on one side of the cooling bin, and an output table is arranged on one side of the cut-off bin. The pipe cutting-off device has the advantages that the conveying rollers are arranged in the cutting-off bin and can drive pipes to pass through the cutting-off bin, the length of the cutting-off bin is limited so that the cut-off pipes can be kept in the conveying state under the action of at least one conveying roller, and the first electric push rod is arranged so that the height of the cut-off pipes can be adjusted to a certain degree; different pipes are different in flexibility, certain bending possibly exists in the conveying process, a first electric push rod is arranged so that the first electric push rod can conduct corresponding fine adjustment on the height of a limiting ring, the pipe conveying process is smoother, the surfaces of the pipes are further cleaned through a cleaning ring, and then the preliminarily cooled pipes are cut off and output through a cut-off blade.

The utility model belongs to the technical field of PPR (pentatricopeptide repeats) pipe fitting production and packaging, and discloses a pipe fitting product leveling device compatible with cartons of two specifications and capable of quickly switching, which comprises an overall frame, a roller conveyor, a leveling mechanism, an adjustable guide limiting mechanism and a sensing device, the leveling mechanism is installed above the overall frame, the adjustable guide limiting mechanism and the sensing device are installed on the two sides of the roller conveyor, the leveling mechanism comprises a leveling air cylinder, a switching air cylinder, a small box leveling pressing block and a large box rapid switching pressing block, the switching air cylinder is fixedly installed above the overall frame, and the switching air cylinder is fixedly installed above the overall frame. The output end of the large box fast switching pressing block is fixedly connected with the upper end of the leveling air cylinder, the small box leveling pressing block is fixedly connected with the output end of the leveling air cylinder, and the large box fast switching pressing block and the small box leveling pressing block are detachably spliced. The device has the function of quickly switching large and small boxes for leveling packaging boxes of bagged pipe fittings, the cost is saved, and the operation is convenient.

The invention discloses a PPR (pentatricopeptide repeats) high-salt-resistant all-potent nuclease mutant and application thereof. The invention firstly discloses an amino acid sequence of the PPR high-salt-resistant all-potent nuclease mutant as shown in SEQ ID NO. 1. The invention further discloses application of the PPR high-salt-resistant all-potent nuclease mutant to removal of nucleic acid. According to the PPR high-salt-resistant totipotent nuclease mutant, alanine at the 165 site of wild PPR high-salt-resistant totipotent nuclease which does not contain signal peptide is replaced with aspartic acid, overexpression can be carried out in a prokaryotic host, and a large number of proteins can be obtained through purification. The PPR high-salt-resistant all-purpose nuclease mutant disclosed by the invention maintains high activity in a wide salt concentration range, a temperature range, a pH range and a Mg < 2 + > range, and can be compatible with most additives used in a biological process. In addition, the activity of the PPR high-salt-resistant all-purpose nuclease mutant is superior to that of other domestic competitive products, and the competitive product level with the best commercial effect at present can be achieved.

The utility model discloses an automatic embedding and taking device for PPR (pentatricopeptide repeats) connected female bent pipe fittings, which comprises an overall device transition mounting plate, and a product taking module for taking out pipe fittings and an embedding module for embedding inserts into a mold are arranged on the lower surface of the overall device transition mounting plate. The insert embedding module comprises a second overturning air cylinder and an insert embedding device mounting plate, the insert embedding device mounting plate is mounted at the end of the second overturning air cylinder, and a set of insert clamping mechanisms used for automatically clamping inserts are arranged on the lower surface of the insert embedding device mounting plate. According to the device disclosed by the utility model, in the injection molding production process of the PPR connected female bent pipe fitting, the injection molding efficiency is improved in two procedures of automatically embedding an insert and automatically taking a product out of the pipe fitting, and meanwhile, the injection molding processing quality is also improved, and the processing period of the injection molding product is shortened.

The utility model discloses a lead-copper-free PPR (pentatricopeptide repeats) anti-freezing fission reducing adapter substitute, and belongs to the technical field of pipeline adapters. Comprising a conversion head body, a first threaded hole is formed in the top face of the conversion head body, a second threaded hole is formed in the bottom face of the conversion head body, a conical cavity is formed in the conversion head body and communicates with the first threaded hole and the second threaded hole, and a connecting block is installed in the middle of the outer side of the conversion head body; connecting rings are installed at the positions, close to the two ends, of the outer side of the adapter body, corrugated pipes are installed on the sides, close to the connecting blocks, of the connecting rings, the corrugated pipes are connected with the connecting blocks in an adsorption mode, heat preservation cotton adheres to the inner walls of the corrugated pipes, and a vacuum cavity is formed in the adapter body; according to the technical scheme, heat loss can be reduced through the heat preservation cotton, the heat preservation effect on fluid in the pipeline is achieved, and the fluid freezing risk is reduced; the vacuum cavity utilizes the vacuum heat insulation principle to further enhance the heat preservation effect and prevent the internal fluid from being frozen due to the too low surface temperature of the adapter body.

The utility model discloses a PPR (pentatricopeptide repeats) cold and hot water pipe connecting piece, which belongs to the field of PPR pipe connecting pieces and is characterized in that sealing equipment is arranged between a sealing seat and a connecting seat, and a butt joint seat and an insertion seat are fixed through bolts; the sealing seat is connected to the inserting seat through a limiting strip, a sinking groove is formed in the upper end of the sealing seat, first sealing filler is injected into the sinking groove, the inserting seat is sleeved with a sealing ring, a cavity is formed in the side wall of the inserting seat, second sealing filler is injected into the cavity, and liquid absorbing sponge is connected to an opening of the cavity. And the butt joint seat and the insertion seat realize sealing and anti-leakage treatment through the sealing seat, the sealing ring and the two groups of sealing fillers. As the sealing seat is easy to replace, the situation that the whole part needs to be replaced due to the fact that the whole connecting piece is damaged can be avoided, and the cost is effectively saved. As a key component in the connecting piece, the performance of the sealing seat is directly related to the sealing performance of the connection. Through the design of convenient replacement, the damaged sealing seat can be found and replaced in time, and then the risk of leakage is reduced.

The invention belongs to the field of polypropylene production processes, and discloses a special material for a PPR (polypropylene random copolymer) pipe in a Spherizone process and a preparation method of the special material. The preparation method comprises the following steps: putting ethylene and propylene into a multi-zone circulation reactor by adopting a Spherizone process, and carrying out binary random copolymerization reaction under the combined action of a main catalyst, a cocatalyst and an electron donor to prepare PPR powder; and degassing and granulating the PPR powder to obtain the special material for the PPR pipe. The special PPR pipe material prepared by the preparation method disclosed by the invention has excellent processability, impact resistance, long-term water pressure resistance and long-term use stability.

The utility model belongs to the technical field of PPR (pentatricopeptide repeats) pipe hot melting, particularly relates to a hot melting device for a PPR pipe, and aims to solve the problems of poor overall operation stability and time and labor waste in the background technology, the following scheme is provided: the hot melting device comprises a hot melting plate frame, and the outer wall of the top of the hot melting plate frame is fixedly connected with symmetrically distributed electric guide rails through bolts; and a fixing frame is mounted on the electric guide rail sliding block. According to the utility model, the positioning rod abuts against the PPR pipe so as to realize quick positioning of the pipe, the bottom jacket is pressed down by the first cylinder so as to clamp the PPR pipe between the bottom jacket and the top jacket, the stability is high, in the process that the electric guide rail drives the fixed frame to move, quick butt joint of the PPR pipe is realized, the butt joint sealing performance is improved, and the production efficiency is improved. Under the mutual cooperation of the handrail, the hanging rings and the universal wheels, the device is convenient to move, has certain portability and is convenient to carry, on the basis, automatic positioning, automatic hot melting and automatic butt joint of PPR pipes can be achieved, the automation degree is high, and time and labor are saved.

The utility model discloses a PPR (pentatricopeptide repeats) pipe fitting for repairing, which is characterized by comprising an arc section pipeline and a straight section pipeline, and one end of the arc section pipeline is fixedly connected and communicated with one end of the linear section pipeline. The pipeline repairing device can improve the efficiency and convenience of pipeline repairing.

The utility model discloses a prefilter machine head structure and a prefilter thereof, which comprise a machine head body, a first pipeline and a second pipeline are arranged on the machine head body, threads are arranged on the first pipeline, the first pipeline is connected with a first PPR (pentatricopeptide repeats) pipe, a nut for connecting the first PPR pipe with the first pipeline is arranged on the outer side of the threads, and the second pipeline is a hot melting joint. The second pipeline is connected with a second PPR pipe, and the second PPR pipe is fixedly connected with the hot melting connector in a hot melting mode. In the installation process, the action of screwing a nut on one side for pressing is reduced, the process of installing an insert is reduced, and the assembly of the pre-filter is simpler.

The invention provides an OsPPR035 gene which is separated and cloned from rice DNA (Deoxyribose Nucleic Acid) and is related to rice stress resistance. The protein encoded by the gene contains a conserved structural domain of a PPR (pentatricopeptide repeat) family, and participates in RNA (Ribonucleic Acid) editing of mitochondrial genes. The expression of the OsPPR035 gene responds to osmotic stress and salt stress to be down-regulated. According to a knockout mutant of the OsPPR035, the RNA editing efficiency of the two loci of the mitochondrial genes rps4-406 and orfX-926 is reduced, the proline content of a rice plant is increased, and the drought resistance and the salt resistance of rice can be improved. The rice OsPPR035 gene has obvious response to adversity stress, the knockout mutant of the rice OsPPR035 gene can enhance drought resistance and salt tolerance of rice through an osmotic regulation mechanism, and the rice OsPPR035 gene can be applied to improvement of adversity resistance of cultivated rice and cultivation of water-saving and drought-resistant rice.