The invention relates to the technical field of
nucleic acid detection, and discloses a method and device for detecting and distinguishing
radix trichosanthis, poria cocos and radix puerariae and terminal device.The method comprises the steps that
genome DNA of a to-be-detected sample is extracted, a specific
DNA fragment is amplified through a universal amplification primer and a specific amplification primer, and an amplification product is obtained; the method comprises the following steps: respectively constructing independent detection modules aiming at
radix trichosanthis, poria cocos and radix puerariae, wherein each independent detection module comprises Cas12a
protein, a
test probe and specific crRNA; the specific crRNA is obtained by screening according to a PAM sequence and a detection
contrast ratio. According to the invention,
genome DNA of a to-be-detected sample is extracted, a universal amplification primer and a specific amplification primer are utilized, then an amplification product is added into an independent detection module, and specific crRNA is utilized to carry out specific recognition on the amplification product, so that a molecular detection
system aiming at
radix trichosanthis, poria cocos and radix puerariae is established, and effective distinguishing of different traditional Chinese medicinal material components is realized.