The invention provides a human respiratory syncytial virus antigen detection method based on magnetic separating and quantum dot labeling. The method comprises the steps that 1, immune nano magnetic beads resisting a human respiratory syncytial virus are prepared; 2, quantum-dot-labeled nano-probes resisting the human respiratory syncytial virus are prepared; 3, after a sample to be detected is dissolved with a sample processing solution, the immune nano magnetic beads resisting the human respiratory syncytial virus are added in a dissolved solution, magnetic separating is performed after full mixing and reacting are performed, washing is performed with a PBST buffer solution, the quantum-dot-labeled nano-probes resisting the human respiratory syncytial virus are added in obtained precipitates, magnetic separating is performed after reacting is performed, and after washing is performed with the PBST buffer solution, a fluorescence value is detected with a fluorescence microplate reader. Accordingly, the accurate, rapid and high-sensitivity method for detecting the human respiratory syncytial virus is established, and the very high practical value in the aspects such as clinical diagnosis, etiology identification and epidemiological investigation of the human respiratory syncytial virus is achieved.