The invention relates to a eucalyptus pruning knife. The eucalyptus pruning knife is composed of a knife holder, a knife handle connecting ring, a handle and a handle connecting ring, wherein the knife holder is divided into a blade I and a blade II. When the eucalyptus pruning knife is applied, the blade I can be used for drawing and cutting downward, the blade II can be used for pushing and cutting upward, tear phenomenon can not be caused when the blade II is applied for pushing and cutting upward and pruning and removing branches and piles, pruning effect is improved, and forest quality is comprehensively improved. Meanwhile, in a link that the eucalyptus pruning knife is applied for pushing and cutting the branches and piles upward, an operator does not need to exert oneself in positive direction but can easily remove the branches and piles, so that the operator can complete pruning and equating operations only by virtue of an upward action a downward action, labour force can be greatly reduced, and pruning cost is also reduced. Besides, when the eucalyptus pruning knife is applied for pruning, non-knot timbers of a eucalyptus are not produced, taperingness is small, fewer knots are produced, texture is uniform, trees are not injured, grow healthily and are good in growth vigour, the whole growth delayed effect of the eucalyptus is enough, outturn percentage is high, and the whole production is more than 15% higher in comparison.