A method of initializing a spectral Doppler mode of operation for an ultrasonic system includes acquiring ultrasound-based data during a two-dimensional mode of operation for a particular examination session and includes automatically establishing settings for the Doppler mode operation parameters based upon the ultrasound-based data. That is, the ultrasound-based data is processed during the session to select spectral Doppler mode settings that are specific to the ongoing session. The session-specific settings are invoked when the system is switched to the spectral Doppler mode. If the two-dimensional mode is a colorflow mode, the Doppler sample volume can be based upon detecting the location of maximum velocity in the colorflow image, the angle correct setting can be based upon maximum velocities in colorflow vectors, the pulse repetition frequency setting can be based upon the maximum frequency shift detected in the colorflow data, and the gain setting can be based upon the amplitude of colorflow data. On the other hand, if the two-dimensional mode is a power mode, the Doppler sample volume is based upon detecting the region of the power mode image having the strongest signals, the angle correct setting can be based upon detecting the direction of flow, the Doppler pulse repetition frequency can be based upon a scale setting for the power mode data acquisition, and a gain setting can be based upon the amplitude of power mode data. Lastly, if the two-dimensional mode is the grey-scale imaging mode, the selection of a Doppler sample volume can be based upon identifying a dark region near the center of the grey-scale image, the angle correct setting can be based upon the orientation of the boundaries of the identified dark region, and the gain setting can be based upon the amplitude of image data.