The invention discloses an efficient corn inbred line combining ability determination
field trial design method, which comprises the following steps: S1, field
layout design: adopting a chessboard type matrix
layout, and planting test seeds and to-be-tested inbred lines in a vertical crossing manner; wherein the n backbone inbred lines serve as test seeds and are planted in rows in the east-west direction, and one test seed is planted in each row; the m to-be-tested excellent inbred lines are planted in a north-south row, and one to-be-tested inbred line is planted in each row; according to the method, the line-column coordinate marks are combined to replace a handwritten
label, so that the risks of missing hanging and wrong hanging are eliminated, and the
test error rate is reduced by more than or equal to 90%; a test seed and a to-be-tested line are planted adjacently, the
pollination path is shortened by 60%-80%, and the single combination
pollination time is shortened from 30-40 seconds of a traditional method to 10-15 seconds; after the process is simplified, one person can independently complete
pollen collection,
pollination and recording, and the labor cost is reduced by 50%; foreign
pollen is effectively isolated through the protection line + bagging design, bagging and
castration are conducted in time before the to-be-detected line
spins and looses
pollen,
selfing is avoided, and the combining ability measuring accuracy is improved by 95% or above.