The invention relates to a needle mushroom root cutting device. The needle mushroom root cutting device comprises a machine frame, a transmission mechanism and a cutting mechanism, wherein the transmission mechanism and the cutting mechanism are arranged on the machine frame; the machine frame comprises a horizontal outer frame, stand columns which are vertically fixed at the lower ends of the four corners of the outer frame and two supporting plates which are horizontally fixed in the outer frame; the transmission mechanism comprises a driving belt wheel, a driven belt wheel, a conveying belt, a plurality of clamps and a first driving motor, the driving belt wheel and the driven belt wheel are horizontally arranged on the two supporting plates correspondingly, the conveying belt is woundaround the peripheries of the driving belt wheel and the driven belt wheel, the plurality of clamps are fixed on the outer surface of the conveying belt at intervals, and the first driving motor drives the driving belt wheel to rotate; and the driving belt wheel and the driven belt wheel are connected to the two supporting plates through vertical shafts in a rotating mode correspondingly, the clamps are detachably provided with the needle mushroom in a clamped mode, moreover, the root of the needle mushroom penetrates out of the lower portions of the clamps, the cutting mechanism is located onone side below the conveying mechanism, the conveying mechanism conveys the clamps provided with the needle mushroom in a clamped mode to one side of the cutting mechanism, the cutting mechanism comprises a horizontal circular blade and a second driving motor for driving the circular blade to rotate, and a cutting edge of the circular blade is used for cutting the root of the needle mushroom below the clamps.