The invention discloses an adhesive film, a preparation method of the adhesive film and a shoe sole comprising the adhesive film, wherein the adhesive film is composed of the following components in the following parts by weight: EVA, maleic anhydride grafted SBS rubber, maleic anhydrided polybutadiene , liquid polyisobutylene, tackifying resin, zinc stearate, cross-linking agent, antioxidant; The adhesive film of the present invention is a cross-linking type adhesive film, and the cross-linking agent decomposes in the molding process to generate active free radicals, Free radical crosslinking occurs between EVA, maleic anhydride grafted SBS rubber, maleic anhydrided polybutadiene and saturated hydrocarbons in the molecular structure of liquid polyisobutylene in the film to form a network structure and improve the film Cohesion; at the same time, the saturated hydrocarbon structure in the film and the saturated hydrocarbon structure in the EVA midsole undergo free radical crosslinking, and the saturated hydrocarbon in the film and the saturated hydrocarbon structure in the thermoplastic rubber backsheet undergo free radical crosslinking, enhancing Improve the interaction force between the film and the EVA midsole and thermoplastic rubber backsheet, that is, improve the bonding strength.