The invention belongs to the field of medicine packaging box processing, and particularly relates to a post-printing processing process for a high-speed mechanical packaging medicine packaging box. The post-printing processing process comprises a die cutting mark pressing process and a box pasting processing process, wherein the die cutting mark pressing process comprises the steps of cutter ranging, proper mark pressing width and die cutting steel wire height determining, plate mounting, pressure regulating, rule determining, rubber mark pressing strip pasting, test pressing die cutting, formal die cutting, waste cleaning and checking, then, the box pasting processing work is carried out, the temperature of the box pasting operation environment is controlled to be 20 to 25 DEG C, the relative humidity is controlled to be 60 percent to 70 percent, paper feeding is carried out, a third folding line of a paper board is enabled to be positioned in the center position of a box pasting machine, the deflection of the pasting opening of a paper box is prevented, pre-folding, gluing, folding, pressuring and compacting are carried out so that the paper box is firmly pasted, the paper box degluing is prevented, and the processed medicine packaging box is collected. The processing process provided by the invention has the advantages that the box type after the box forming is regular, the box pasting is firm, the quality of the medicine packaging box is improved, the machine halt rate in a high-speed mechanical packaging line can be greatly reduced, and the satisfaction degree of customers is improved.