The invention discloses an automatic multi-shot plastic blood vessel serrefine and a serrefine case. The automatic multi-shot plastic blood vessel serrefine comprises a serrefine mouth mechanism, a case containing mechanism, an automatic serrefine delivery mechanism, a serrefine applying mechism and a handle mechanism. By means of the automatic multi-shot plastic blood vessel serrefine and the serrefine case, the operation speed can be obviously and effectively improved, namely a doctor only needs to put an automatic multi-shot applied serrefine into a human body in one time to perform multiple-time continuous serrefine applying and does not need to take an instrument from the human body in the operation process. In addition, the operation time waste for instrument transfer between the operation doctor and a nurse and installation is avoided by means of the structure, operation risk is reduced, the multi-shot plastic blood vessel serrefine is capable of performing opening-closing motion and can be inversely buckled to achieve self locking, a large blood vessel ligation and closing are easy, blood vessel alignment is easier, operation is more convenient to perform, the closed blood vessel is firm, and quality is improved obviously. Furthermore, the automatic multi-shot plastic blood vessel serrefine can be disinfected for multiple times and repeatedly used, the serrefine case is disposable, and therefore product cost is low.