The invention provides a sterile isolation device for a laparoscopic surgical robot and the laparoscopic surgical robot thereof, and relates to the field of laparoscopic surgical robots, the sterile isolation device comprises a robotic arm, an execution arm, a non-elastic transparent film, a connector and an instrument, the execution arm is connected to the robotic arm, the instrument is connected to the execution arm through the connector, and the non-elastic transparent film is arranged on the non-elastic transparent film. And one end of the inelastic transparent film is sleeved on the mechanical arm, and the other end of the inelastic transparent film is adhered to the connector, so that the mechanical arm, the execution arm and part of the connector are sleeved in the inelastic transparent film. Rapid installation and replacement of all parts are achieved through the quick-change switch, and usability is achieved; absolute sealing is achieved through the inelastic transparent film, and safety is achieved; the non-elastic transparent film is common in material, easy to manufacture and economical, on one hand, interference of an elastic body on the motor is avoided, on the other hand, absolute sterile isolation is achieved, and the safety of an operation can be greatly improved.