The invention discloses a super-resolution
microscopy method based on dual-mode competition stimulation and a super-resolution
microscopy device based on dual-mode competition stimulation. The method comprises the following steps of: irradiating the surface of a fluorescent sample by use of two types of modulated light beams emitted from a same
light source, wherein one
light beam is focused to form high-energy hollow
light spot on the sample after passing through a
spatial light modulator, so as to generate a saturation effect, and the other
light beam is modulated into pulsed light by an acousto-optic modulator so as to be focused to form low-energy
solid light spots on the sample; and collecting
signal lights emitted from each scanning point of the sample, and extracting the
signal lights which have same frequency with the pulsed light, wherein the extracted
signal lights serve as effective signal lights for acquiring super-resolution images. The device is simple, and convenient to operate; by use of the competitive mechanism of the
solid light spots and the hollow light spots during a process of stimulating sample fluorescent, the resolution ratio for saturated
fluorescence to stimulate an ultra-
diffraction limit is realized by only using one
laser light source.