The invention discloses latex for waterborne interior-wall primer and a preparation method and application thereof. The latex comprises, by weight, 22.1-29.9% of monomer, 1.1-2.3% of polymerization partitioning agent, 0.04-1.05% of stabilizers, 0.1-0.5% of plasticizers, 0.1-0.25% of initiators and 66.1-75.0% of solvent, wherein the monomer is a mixture of vinyl acetate, ethyl methacrylate, butyl acrylate, isooctyl acrylate and acrylamide, and the polymerization partitioning agent is a mixture of pyrrole and morpholine. The prepared latex is good in closure performance, high in adhesive force and infiltration capacity, highly resistant to alkali burnout and water, good in watertightness, outstanding in salting-out resistant effect, remarkable in interlayer collocation effect and capable of effectively avoiding the defects of poor lasting effect, instable interlayer collocation, failure in reaching the standard of VOC (volatile organic compounds) and the like when common latex is used for the primer, thereby being a good binder for preparing excellent waterborne primer.