The invention relates to a preparation method of a
graphene microsphere biological separation medium with a controllable particle size. The preparation method comprises the following steps: uniformly stirring and mixing
graphene oxide and deionized water to obtain a
mixed solution; performing ultrasonic treatment and cooling on the
mixed solution, then atomizing into
aerosol droplets, passing the
aerosol droplets through a
quartz tube, collecting by using a
polytetrafluoroethylene (PTFE)
filtration membrane, and
drying to obtain
graphene microspheres; and preparing a graphene
microsphere separation medium taking Reactive Blue 4 as a ligand. According to the method provided by the invention, the affinity separation medium is used for separating and purifying a lot of
papain from papaya
powder, testing the enzymatic activity and the
protein content, and calculating purification folds; and the method provided by the invention is quick, simple and convenient, is large in separation quantity, good in activity and high in purity of an extracted
enzyme, and is suitable for scale production.