The application belongs to the technical field of
cell culture, and particularly relates to a method for determining the number of particles in a
unit volume of liquid and application. The method comprises the following steps: (1) using a dye to dye the liquid to be measured, and sequentially dropping the dyed liquid to be measured and a
chromatographic separation liquid at one end of
filter paper, the particles in the liquid to be measured are dispersed on the
filter paper under the action of capillary
chromatography, and the number of particles in the liquid to be measured is obtained by naked-eye counting; (2) according to the volume of the liquid to be measured, the number of particles in a
unit volume of liquid is calculated. The determination method of the application does not need to purchase expensive instruments, does not need to take photos and use
image analysis software for auxiliary counting, breaks through the limitation that a
blood cell counter cannot determine particles with a particle size greater than 100 mu m, and can complete the determination of the number of particles by naked-eye observation, which is simple, efficient and low in cost.