The invention relates to a trephine having a limiting sleeve, wherein the trephine is used for a medical surgery purpose in skull defect and skull puncture surgeries. The trephine with the limiting sleeve comprises a motor, an internal trephine, and at least one trephine sleeve. The internal trephine is nested in the trephine sleeve by means of at least two bearings and is driven, by means of themotor, to rotate. The external trephine sleeve defines and adjusts the cutting depth of the internal trephine, while protecting a surgery operator and the tissue around a surgical site from being injured by a trephine rotating at a high speed; moreover, by drilling using the trephine having a limiting sleeve, a bone flap can be completely removed and retrieved from a trephined bone. The present invention realizes standardization and repetition of cranial defect and drilling surgery, and makes the surgery simple, easy to operate, safe and reliable, efficient, and is less traumatic. In a commonmedical researching laboratory condition, the trephine supplies a surgery technological insurance for a skull defect model and a clinical medical craniotomy and skull puncture of mice to large animals.