47 results about "Coldwater fish" patented technology

The invention relates to a technical method of artificial propagation of rainbow trout, belonging to the technical field of coldwater fish (salmon and trout) cultivation. The technical method comprises the following steps: step 1, preparing seepage of single salt and the like; step 2, implementing egg washing and artificial insemination of seepage of single salt and the like; step 3, implementing continuous operation of flowing water, absorbing water, expanding and incubation of oosperm in a mesh pot barrel-type incubator; step 4, detecting fertility rate in 4 cell stage; step 5, adopting a mesh cage (or gravel) flat flow type larval fish film-out incubation groove to implement larval fish film-out incubation; step 6, after the larval fish is incubated, removing the mesh cage or the gravel, carrying out larval fish opening training and raising in a primary groove; step 7, after one month of training and raising, removing the larval fish into a hexagonal raising groove, and carrying out offspring seed raising. By adopting continuous operation technology of flowing water, absorbing water, expanding and incubation, the technical method reduces operation procedures and operation tools of stopping water, absorbing water and expanding, and improves the incubation rate by 20%. Compared with the existing embryonic detection technology, the fertility rate detection technology in 4 cell stage is mastered 9 days in advance, thus avoiding the problem that embryonic detection is not accurate.

The invention provides a cold water fish forage. The forage comprises raw materials of, by weight: import fish meal 14-16 parts, domestic fish meal 7-9 parts, yeast powder 3-5 parts, soybean phospholipids 3-5 parts, soybean meal 28-32 parts, peanut meal 16-18 parts, chicken blood meal 2-3 parts, cinnamon meal 1-2 parts, epimeddium meal 2-3 parts, spirulina meal 4-6 parts, wheat gluten meal 5-7 parts, fish oil 1-2 parts, calcium lactate 0.4-0.6 parts, calcium dihydrogen phosphate 2-3 parts and rhizoma polygonati meal 0.02-0.04 parts. The cold water fish forage is prepared with a conventional puffed fish forage preparation method by puffing granulation, drying, oil injection and cooling. The cold water fish forage is anecological environment-friendly green fish forage; when fish is fed with the cold water fish forage provided by the invention, a use ratio of the cold water fish forage is high; antiviral ability of fish is strengthened; a growth rate of fish is increased; a survival rate of cold fish is increased; and culture benefit is increased.

The invention belongs to the technical field of development of aquaculture and ocean resource, and relates to a device and method for catching cold water fish in a large aquaculture net cage in summer. The device comprises a floating frame, a fish collecting part and a fish guiding channel which are connected in sequence, the floating frame is fixedly arranged on an upper half portion of the fish collecting part, and a lower half portion of the fish collecting part is connected with the fish guiding channel; a light inducing device is arranged in the fish guiding channel; during use, high-temperature water in the fish collecting part is pumped out through a water pump, such that cold water at a bottom layer enters the fish collecting part; fish schools are induced to enter the fish collecting part through bait and the light inducing device; after the fish schools in the fish collecting part reach a certain density, a connecting part is closed and fish in the fish collecting part is captured; and after fishing is completed, the device is removed. The provided device and method are used for fishing, the device is removed after fishing is completed, and normal culture in the net cage can be recovered. The device is simple, the method is easy to implement, a physiological mechanism and behavioral characteristics of fish are fully considered and utilized, and a problem of catching cold water fish cultured in warm sea areas in summer is solved.

The present invention relates to a method for detecting a pathogen in coldwater fish using insulated isothermal polymerase chain reaction (iiPCR). In addition, the present invention also relates to pairs of oligonucleotides for detecting pathogens in coldwater fish.

A slowly-releasing hormone for cold-water fish contains water, gelatin, gonad stimulating hormone or ganodotropic releasing hormone or leuteiniznig hormone, etc.

The invention relates to a first feeding feedstuff of taimen. The taimen is a famous and precious large-sized coldwater fish, and the domestication and the cultivation of the taimen are successful andare popularized across China. However, the feedstuff for the first feeding stage of the taimen does not exist, the taimen is only fed with biological baits such as tubificidae, but the biological baits have the problems of unstable quality, insufficient supply of goods, seasonality, carried disease causes, outbreaks of diseases frequently, high transit death rate and the like, and influence the domestication effect of the first feeding stage of the taimen. The first feeding feedstuff comprises the components of dextrin, full cream milk powder, blood meal, fish meal, soy protein isolate, soybean lecithin, fish oil, fish vitamins, fish microelements, choline, beet, hydroxymethyl cellulose sodium and enzyme preparation. The first feeding feedstuff is applied to the feeding of the taimen at the first feeding stage.

The invention discloses a treatment method and system for cold water fish breeding water in northern mountainous areas. The method comprises water source purification and wastewater purification. A water source purification process comprises the following steps that: river water is pumped by a water pump, flows through a first filter screen, enters a first physical treatment area with zeolite andanthracite as media, and is disinfected by an ultraviolet sterilizer. A wastewater purification process comprises the steps that breeding wastewater enters a biological treatment area through a secondfilter screen, is treated with a microbial agent, then enters a second physical treatment area with slag as a medium for treatment and then is discharged, wherein the second physical treatment area is covered with soil having a thickness of 20-30 cm to plant emergent aquatic plants. By means of the method, impurities and harmful substances in a water source can be effectively removed, a clean water source can be continuously provided for cold water fish breeding; and in addition, pollution of wastewater generated after cold water fish breeding to a water body can be reduced by purifying breeding wastewater, water quality is improved, and technical support is provided for purification and restoration of the water body.

The invention relates to a special nutrition enhancer for parent fishes of cold water fishes and a use method of the special nutrition enhancer, and belongs to the technical field of aquaculture. According to the genders of the parent fishes, the enhancer is divided into a special nutrition enhancer for male parent fishes and a special nutrition enhancer for female parent fishes; the special nutrition enhancer for the male parent fish comprises a special nutrition enhancer for section-I male parent fishes and a special nutrition enhancer for section-II male parent fishes; and the special nutrient enhancer for the female parent fishes comprises a special nutrient enhancer for section-I female parent fish, a special nutrient enhancer for section-II female parent fishes and a special nutrientenhancer for section-III female parent fishes. Different raw material formulas are selected according to the growth characteristics of the parent fishes in different time periods, and nutrient enrichment is carried out at different times, so that the nutrient state of the parent fishes of the cold water fishes is improved. The special nutrition enhancer has the advantages of scientific formula, reasonable process, convenience in use and strong pertinence, supplements special nutrient elements in a breeding production process of the parent fishes of the cold water fishes, and improves the reproductive capacity of the parent fishes by adding functional substances.

The invention discloses an artificial propagation method of coregonus nasus. The method comprises the steps of parent fish culture, parent fish inspection, artificial egg collection and insemination,oosperm quality identification, artificial incubation and fry pond culture, and coregonus nasus fries are successfully propagated. According to the method, the success of the artificial propagation technology of the coregonus nasus is achieved for the first time, and the coregonus nasus fries are obtained and cultured through a pond culture method; and the culture effect of the coregonus nasus fries is improved, the fry culture cost is reduced, practical significance is achieved in solving the supply problem of the coregonus nasus fries, meanwhile, the problems that the current coregonus nasusculture varieties in the Xinjiang region are few, the fries depend on import, and the culture period is long can be effectively solved, the coregonus nasus exerts greater economic and social benefits, and the method is of great significance to the development of Xinjiang cold water fish industry.

The invention relates to a coldwater fish high-density hatcher which comprises a hatching trough, hatching boxes, a water inlet tube and a water outlet tube, wherein the hatching boxes are arranged in the hatching trough side by side, and the water inlet tube and the water outlet tube are communicated with the hatching trough; the water inlet tube and the water outlet tube are respectively arranged at the front end and tail end of the hatching trough; the bottom plate of each hatching box is provided with permeable holes, and the rear side plate of each hatching box along the water flow direction is provided with water outlet holes and a hatching box baffle fixedly connected with the hatching box; and each hatching box is internally sleeved with a sieve tulle. The coldwater fish high-density hatcher has simple structure and low construction cost, can carry out high-density hatching, occupies small production space, saves energy, is easy to manage and is simple and convenient to operate; 500-1000 g of oosperms can be placed in each hatching trough; and 5-year experiments prove that the hatching rate is above 70%, and the average hatching rate of the oosperms can be more than 85% when the weight of the oosperms in each box is 700-800 g.

The invention discloses a device and method for preventing icing from damaging a wading building. The device comprises a fish culture net cage which is rectangular in shape and is arranged adjacent tothe wading building, the fish culture net cage is fixedly arranged on the wading building through a fixing structure, the lower part of the fish culture net cage extends into a water body below an ice layer, and the upper part of the fish culture net cage is exposed out of the top end surface of the ice layer above the water body; and cold water fish with certain density and used for driving water in the fish culture net cage to perform heat exchange with the water below the ice layer by utilizing self-swimming in winter so as to prevent icing of the wadding building is bred in the fish culture net cage. According to the device and method for preventing icing from damaging the wading building, a biological method is adopted to avoid damage of icing to the wadding building, electric powerand other energy sources are not consumed, the fish culture net cage is arranged around the wading building, and the cold water fish is bred in the fish culture net cage, so that the cold water fish drives water in the fish culture net cage to exchange heat with the water below the ice layer by using self-swimming in winter, and the purpose of preventing the wadding building from icing is achieved.

The invention relates to a coldwater fish high-density hatcher which comprises a hatching trough, hatching boxes, a water inlet tube and a water outlet tube, wherein the hatching boxes are arranged in the hatching trough side by side, and the water inlet tube and the water outlet tube are communicated with the hatching trough; the water inlet tube and the water outlet tube are respectively arranged at the front end and tail end of the hatching trough; the bottom plate of each hatching box is provided with permeable holes, and the rear side plate of each hatching box along the water flow direction is provided with water outlet holes and a hatching box baffle fixedly connected with the hatching box; and each hatching box is internally sleeved with a sieve tulle. The coldwater fish high-density hatcher has simple structure and low construction cost, can carry out high-density hatching, occupies small production space, saves energy, is easy to manage and is simple and convenient to operate; 500-1000 g of oosperms can be placed in each hatching trough; and 5-year experiments prove that the hatching rate is above 70%, and the average hatching rate of the oosperms can be more than 85% when the weight of the oosperms in each box is 700-800 g.

The present invention relates to a method for detecting a pathogen in coldwater fish. In addition, the present invention also relates to pairs of oligonucleotides for detecting pathogens in coldwater fish.

The invention provides a feed additive for improving the fish quality of cold water fish and an application of the feed additive. The additive comprises the following components in parts by weight: 15-30 parts of cortex eucommiae, 10-25 parts of bighead atractylodes rhizomes, 10-30 parts of dioscorea opposita, 5-15 parts of radix isatidis, 10-20 parts of radix angelicae sinensis, 5-10 parts of licorice roots, 0.5-1 part of red lotuses and 0.1-0.5 part of roots, leaves and fruits of rhus chinensis. According to the feed additive disclosed by the invention, the meat quality of the cold water fish can be improved, and the meat quality of the cold water fish can be delicious; the red lotuses and the roots, leaves and fruits of rhus chinensis can avoid the additive from influencing the meat quality of the cold water fish, and have the effect of promoting the sense organ effects of the meat quality, the meat quality is tender and smooth and white in color, the growth speed of the cold water fish is increased, the immunity can be enhanced, the digestive capacity and the disease resistant capacity of the cold water fish can be promoted, the utilization rate of a feed can be increased, and besides, stress response to the cold water fish is retarded.

The invention provides a full-submersible closed culture fishing ground. The ground comprises a culture tank, a buoyancy cabin, a circulating water pump, a wave energy power generation device and a material replenishment and supply buoy platform. A water inlet pipe capable of going deep into a deep cold water layer is arranged at the bottom of the culture tank. The buoyancy cabin is arranged at the periphery of the bottom of the culture tank; the circulating water pump is communicated with the interior of the culture tank; the wave energy power generation device is connected with the circulating water pump and supplies power to the circulating water pump; and the material replenishment and supply buoy platform floats on the water surface and is connected with the culture tank through a hose. The technical problems that according to a net cage culture manner in the prior art, culture water is the same as external water, cold water cannot be kept in a culture area, and the cold water fish culture industry cannot be developed are solved. Circulating exchange between warm water in the culture tank and cold water in the external deep water layer is achieved, it is guaranteed that the interior of the culture tank is a cold water body, and therefore the technical effect that large-scale cold water fish culture is conducted in a warm heat zone water area through marine resources in China is achieved.

The present invention relates to the use of alpha lipoic acid as feed additive for aquatic animals including fish and shrimp, especially for cold water fish as for example salmon, bream, bass and forwarm water fish as for example carp, tilapia, catfish. More particular, this invention relates to the use of alpha lipoic acid for the improvement of the feed conversion ratio and / or daily weight gainin fish, for reducing mortality by regulating the micro flora of the gut and / or by protecting the animal against infections caused by pathogenic viruses.

The invention discloses a feed additive composition for cold water fish. The feed additive composition comprises the following raw materials in parts by weight: 15-25 parts of radix isatidis, 1-5 parts of endothelium corneum gigeriae galli, 20-30 parts of scutellaria baicalensis, 10-20 parts of radix rubiae, 10-20 parts of parasitic loranthus, 15-25 parts of bighead atractylodes rhizome and 5-10 parts of glossy privet fruits. The feed additive composition disclosed by the invention can be added in feed by a dosage of 5-10%, and has the advantages that the health of the cold water fish can be protected in the culture process of the cold water fish, the immunity can be improved, the food intake, body weight and survival rate of the cold water fish can be increased, and the economic loss can be avoided.

The invention discloses a ball-rod double nanostructure enhanced cold water fish gelatin-based edible film and a preparation method and application thereof. The method comprises the following steps: 1) dropwise adding a calcium chloride solution into a sodium alginate solution to obtain calcium alginate pregel, dropwise adding a chitosan hydrochloride solution into the pregel, performing ultrasonic treatment and centrifugation to obtain supernate, and freeze-drying to obtain spherical nanoparticles; 2) soaking and cleaning bagasse, drying, bleaching, adding acid for hydrolysis, centrifugally dialyzing to be neutral, and freeze-drying to obtain cellulose nanocrystals; (3) adding a sorbitol-glycerol plasticizer into the ball-rod double nanostructure enhanced cold water fish gelatin solution, uniformly stirring, and carrying out ultrasonic degassing; 4, treating the coating solution through a tape casting method to obtain the edible film, and meanwhile, improving the performance of the edible film through magnetic treatment. The prepared cold water fish gelatin-based edible film can be used for reducing cracking of cherries in rain, has biodegradability and is safe and non-toxic.

The invention discloses a Chinese herbal medicine-based feed additive combination for cold-water fish and a preparation method and a use thereof. The Chinese herbal medicine-based feed additive combination for cold-water fish is composed of the following raw materials by weight: 10-20 parts of liquorice, 10-20 parts of garlic, 10-20 parts of acalypha australis, 5-10 parts of Chinese gallnut, 10-20 parts of Chinese mugwort leaf, 5-10 parts of vervain, 1-5 parts of gecko, and 5-10 parts of cinnamon. The Chinese herbal medicine-based feed additive combination for cold-water fish can be added to the feed according to the dose of 5-15%. The Chinese herbal medicine-based feed additive combination for cold-water fish has an obvious sterilization and disinfection function, and can effectively improve the disease resistance of cold water fish so as to improve the survival rate thereof.

The invention belongs to the field of cold water fish culture, and relates to a mountainous area cold water fish semi-circulating water culture system with a self-cleaning function and a utilization method. The mountainous area cold water fish semi-circulating water culture system comprises a cold water fish culture pond, a water body ecological purification pond, a nanotube air lift water circulation device, a composite water purification device, an ecological floating bed and a water baffle, a separation wall is arranged between the cold water fish culture pond and the ecological purification pond, pond tails are communicated, and the mountainous area cold water fish semi-circulating water culture system comprises a water inlet located in the culture pond and a water outlet located in the water body ecological purification pond. The composite water purification device comprising a hydroponic planting plate and a biochemical brush is erected in the ecological purification pond and is matched with the ecological floating bed, and pollutants in the water body are decomposed and absorbed by culturing low-temperature-resistant microorganisms and planting low-temperature-resistant aquatic plants, so that water body ecological purification is realized. The nanotube air lift water circulation device efficiently oxygenates the ecologically purified water body and then pushes the water body into the cold water fish culture pond, and semi-cyclic utilization of the water body is achieved. Meanwhile, the water outlet of the ecological purification pond is provided with a drainage device capable of adjusting the drainage amount, the drainage amount of the water body after ecological purification is increased in the wet season, the drainage amount is reduced in the dry season, the air lift water circulation amount is increased, so that the problems of insufficient water amount and dissolved oxygen in the dry season are solved, and standard discharge of cold water fish culture tail water can be realized, so that the green development of the cold water fish culture industry is promoted.

The invention provides a floating weight for playing water and floating games and a preparation method thereof. Compound; mix fish meal, bran, bean cake, crush, yeast powder, multivitamins, salt, vegetable oil, methionine, lysine, valine, sodium glutamate, sodium alginate and minerals, stir and mix, and then mature Granulate, dry, and then use calcium chloride to obtain hardened feed; knead the compound into a flat skin to wrap the hardened feed, wrap it into a spherical float embryo, and then place it in a spherical mold cavity to form it. Then dry to obtain a spherical-shaped floating weight for playing water and drift games, wherein the angle between the inner arc surface of the mold cavity and the bottom surface of the mold cavity is 18-22°, and the mold cavity is smooth. The water drift game drift weight prepared by the invention has good water drift effect and can be used as cold water fish feed without recycling.

The invention discloses a cold-water fish probiotic Lactococcus lactis strain and its application. The present invention isolates and identifies a strain HZC32 that has antiviral properties, immune regulation and immune function enhancement properties to cold water fish from the intestinal mucosa of semi-wild rainbow trout juveniles in the Benxi mountainous area of ​​Liaoning Province. According to the observation of the morphological characteristics of the colony and the determination of the 16SrDNA base sequence and homology analysis, the strain identification result is: HZC32 belongs to Lactococcus lactis, and the preservation number is: CGMCC No.10705. The in vitro cell experiments proved that: Lactococcus lactis HZC32 strain co-cultured with virus-infected EPC cells has the property of resisting IHNV proliferation in EPC cells in vitro. Animal experiments have proved that: Lactococcus lactis HZC32 strain has the characteristics of immune regulation and immune function enhancement to cold-water fish, and the HZC32 strain fed alone also has the characteristics of anti-IHNV infection to cold-water fish. Thus it can be seen that the Lactococcus lactis HZC32 strain of the present invention has a certain application prospect in cold-water fish anti-virus, immune regulation and enhancement of cold-water fish immune function.

The invention discloses a cold water fish culture pond feeding device, and belongs to the technical field of cold water fish culture. The cold water fish culture pond feeding device comprises a bearing box, a conveying shell, a first motor, a second motor, a baffle plate, a conical block, a spiral conveyor, a slide rod and a slide sleeve; the upper side and the right side of the conveying shell are installed on the upper surface and the right side face of the bearing box correspondingly, the first motor is installed on the upper surface of the inner wall of the bearing box, an output shaft ofthe first motor is fixedly connected with a threaded column, and the surface of the threaded column is in threaded connection with a threaded cap. According to the cold water fish culture pond feedingdevice, by arranging the first motor, the second motor, a lifting plate, the baffle plate, the spiral conveyor, the conical block and a discharging hopper, the height of the baffle plate can be controlled through work of the first motor, the discharging speed of fodder can be controlled according to the actual situation, the spiral conveyor can evenly convey the fodder, and the conical block canthrow out the fodder, so that cold water fish schools need to be fed dispersedly, and the situation of snatching food is avoided.

The invention discloses a cold-water fish gelatin base film reinforced with ball-rod double nanostructures and its preparation method and application, comprising the following steps: 1) adding calcium chloride solution dropwise to sodium alginate solution to obtain calcium alginate Pre-gel, then add chitosan hydrochloride solution dropwise to the pre-gel, ultrasonically treat, centrifuge to obtain supernatant, and freeze-dry to obtain spherical nanoparticles; 2) Soak bagasse, wash and dry, bleach Add acid hydrolysis, centrifuge dialysis to neutrality, and obtain cellulose nanocrystals after freeze-drying; 3) Add sorbitol-glycerin plasticizer to the cold-water fish gelatin solution reinforced with ball-rod double nanostructures and stir evenly, and degas it by ultrasonic ; 4) The coating solution is processed by casting method to obtain gelatin base film, meanwhile, the film properties can be improved by magnetic field treatment. The cold-water fish gelatin base film prepared by the invention can be used to reduce fruit cracking of cherries in rain, and has biodegradability, safety and non-toxicity.

The invention belongs to the technical field of aquaculture water quality monitoring equipment, and particularly relates to a cold water fish aquaculture water quality and flow velocity real-time monitoring and early warning device which comprises a main body, supporting columns with different use lengths are arranged on the outer side of the main body, and extension columns capable of being fixed to different depths are arranged at the lower ends of the supporting columns. And detection equipment is arranged on the outer sides of the supporting columns and the extension columns. The positioning holes are formed in the supporting columns and the extension columns at equal intervals, the pins are installed in the positioning holes, the extension columns slide to adjust the use length to drive the detection equipment to move up and down to be fixed to different depths, operation is easy, use is convenient, meanwhile, water quality in a deep area can be detected, and the data accuracy is improved; after the extension column is sectionally arranged to be of a telescopic structure, the ring sleeve is closed to press the elastic plate so that the elastic plate can be connected into the clamping base in a clamped mode, the ejection block is used for abutting against the inner end face of the clamping base, the multiple detection devices can be adjusted to different depths, and the water quality of different depths can be detected at the same time.

The invention discloses a nested culture method for cold water fishes and normal-temperature fishes. The method comprises a culture pond, the culture pond is internally provided with a cold water area, a heating bin and a normal temperature area in the horizontal direction, the culture pond is provided with a moving area and an excrement area in the vertical direction, the heating bin is internally provided with a heating plate, and one side of the culture pond is provided with a cooling purification pond. A main flow pipeline is fixedly installed at one end of the cooling purification pond, a booster pump is fixedly installed on the main flow pipeline, a water inlet spray head is fixedly installed at the output end of the booster pump and located on one side of the cold water area, and a transfer collecting barrel is connected to the side, located on the normal temperature area, of the culture pond. A filtering plate is fixedly installed at the central position in the transfer collecting barrel. A sealing cover plate matched with the filtering plate is hinged to the transfer collecting barrel. In the using process, cold water fish and normal-temperature fish nested breeding is adopted, water resources are saved, control is convenient, and the survival rate of the cold water fishes and the normal-temperature fishes is increased.

The invention belongs to the technical field of food preservation, particularly relates to fresh freezing liquid for food sterilization and preservation and a preparation method thereof, and solves the problems that in the prior art, fresh freezing liquid only serves as a heat conduction medium, the heat conduction efficiency is low, a good sterilization effect cannot be achieved in a freezing process, and the food quality and freshness preservation effect is limited. The fresh freezing liquid for food sterilization and preservation comprises the following raw materials of ethanol, sodium chloride, an active ingredient, a synergist, sodium citrate and deionized water, wherein the active ingredient of the fresh freezing liquid is prepared by compounding a plant extract and a cold water fishbone extract; and the synergist is prepared by mixing sea buckthron oil and calcium carboxymethylcellulose in a mass ratio of 1:(1-2). The fresh freezing liquid is scientific in formula, rigorous in proportion, simple in preparation process, low in preparation cost, stable in performance, low in working temperature, low in volatility, low in corrosivity, capable of improving the product quality and prolonging the storage period when being used for food preservation, and free of pollution.