The application discloses a kind of live
cell clathrin imaging parameter optimization method and related device based on SRRF nanoscope, it is related to biomedical imaging field, the method includes under live
cell culture condition, when using TIRF
microscope to the
time series imaging of live
cell sample expressing fluorescent
label, original
image sequence is obtained;It is input NanoJ-SRRF
processing system, a plurality of core reconstruction parameters are systematically combined, and several super-resolution reconstruction images are generated;For the super-resolution image reconstructed by each combination parameter, comprehensive quantitative evaluation is carried out using multimodal
evaluation system, and multimodal quantitative quality
evaluation result is obtained;Based on this result, the parameter combination that meets the preset resolution, structure fidelity index and the minimum
visual artifact is screened out, and the optimal parameter set for the imaging of live cell clathrin coated pit is determined.The application can reduce reconstruction artifact, realize high-fidelity nanoscale observation of live cell CCP dynamics.