The invention discloses a serial
crystallography experiment device based on a
chip technology and an assembling method thereof. The device comprises a
chip, a
chip base, a chip operation board, a chip lower disc, a chip upper disc and a low background scattering film, a plurality of hollow chip holes are formed in the chip and are used for supporting and adsorbing the
crystal; the lower chip disc and the upper chip disc fix the low background scattering films at the upper end and the lower end of the chip base in a nesting manner; a liquid removal chamber groove and a liquid removal chamber are arranged on the chip operation board and are used for removing redundant
crystal solution in the chip; the groove of the liquid removal chamber is used for accommodating a chip base, an air exhaust hole is formed in the side wall of the liquid removal chamber and connected with an air
exhaust pipe of an air exhaust
system, and acting force is generated on the
crystal solution to enable the redundant crystal solution to be sucked to the other end of a chip hole; and the chip operation board is provided with an observation chamber groove and an observation chamber and is used for observing the state of the crystal in the chip hole. According to the invention, the chip can be rapidly assisted to remove redundant crystal solution, and the chip can be rapidly observed, assembled and installed on a line
station.