The invention discloses a transmitted light three-dimensional super depth-of-field microscope with an additional lighting module, which comprises a device main body, a microscope station is arranged at the upper end of the device main body, an electric control box is arranged in the device main body, a positioning bracket, a movable support, a control console and a control key are arranged at the upper end of the microscope station, and the control key is arranged at the lower end of the device main body. A supporting table is fixedly installed at the front end of the positioning support, a lifting support is arranged at the front end of the supporting table, and a positioning support is installed at the front end of the lifting support. According to the transmitted light three-dimensional super-field-depth microscope with the additional illumination module, the microscope support, the microscope control module and the illumination module are integrated, microscope observation can be conveniently and better carried out, the observation effect is more excellent, the use performance of the microscope is improved, the microscope can be conveniently controlled, and the service life of the microscope is prolonged. And the lighting effect is achieved, the shadow condition is prevented, and better use is facilitated.