The invention discloses a manufacturing process of a polyester cotton yarn-dyed fabric. The manufacturing process comprises the following technological steps: selecting a polyester cotton blended yarn; sequentially performing scouring, blanching, dyeing, soap-boiling, water washing and drying, wherein beam dyeing is adopted, and in the dyeing process, the winding density of a warp of a warping machine is 0.46-0.48 g/cm<3>; before dyeing, adding 0.2-0.4 g/L sodium sulfate into water required by dyeing, performing stirring for 8-10 min; adding sodium carbonate first at the water temperature of 35-40 DEG C; after 12-15 min, adding sodium sulfate for 12-15 min; and maintaining for 42-46 min when the temperature rises to 62 DEG C. According to the manufacturing process, the dyeing effect is improved, more uniform dyeing is achieved, the dyeing freshness is guaranteed, the static property of a spun polyester thread is reduced by an anti-static agent, the static ratio of the polyester cottonyarn-dyed fabric which is woven is favorably reduced on the whole, in addition, triple-jump, yarn breakage and yarn sinking are reduced, and the sizing percentage is increased.