30 results about "Cellulose synthetase" patented technology

The invention discloses a plant expression vector capable of effectively interfering a phytophthora capsici cellulose synthase 3 gene through host-induced gene silencing and application of the plant expression vector. The plant expression vector disclosed by the invention can be transcribed to form double-stranded RNA with a reverse complementary structure, and the double-stranded RNA is formed by respectively inserting a nucleotide sequence shown as SEQ ID No.1 or SEQ ID No.2 into the vector twice in the forward direction and the reverse direction. The invention also discloses application of the expression vectors in preventing and treating plant diseases caused by phytophthora capsici. The plant expressing the vector provided by the invention has obviously enhanced disease resistance to phytophthora capsici, the expression of the cellulose synthase 3 gene of the phytophthora capsici infecting the plants is significantly inhibited, and the cellulose synthase 3 gene is necessary for the development and pathopoiesis of the phytophthora capsici. Therefore, the plant expression vector can effectively inhibit plant infection caused by phytophthora capsici, and an effective way is provided for preventing and treating related diseases caused by phytophthora capsici.

This invention relates to an isolated nucleic acid fragment encoding a cellulose synthase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the cellulose synthase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the cellulose synthase in a transformed host cell.

The present application belongs to the technical field of tobacco genetic engineering, and specifically relates to a patent application for a method for detecting the Q1077H mutation site in Phytophthora nicotianae cellulose synthase CesA3. Compared with the wild type, after the cDNA sequence of Phytophthora nicotianae cellulose synthase CesA3 gene was mutated from G to T at the 3231st nucleotide from the 5' end, the resistance of Phytophthora nicotianae to dimethomorph can be determined by the sensitive mutation. Correspondingly, amino acid 1077 from the N-terminus of Phytophthora nicotianae cellulose synthase CesA3 was mutated from wild-type glutamine to histidine. Based on the principle of AS-PCR technology, the inventors designed relevant primers for rapid detection and identification of Phytophthora nicotianae with resistance to dimorpholine after mutation, which can be used to adjust the prevention and control strategies of tobacco black shank, as well as delay and control The further development of drug resistance and the research and development of related new chemical drugs lay a certain technical foundation.

Provided is a recombinant microorganism having enhanced cellulose synthase gene stability, a method of producing cellulose by using the recombinant microorganism, and a method of preparing the recombinant microorganism.

The invention discloses a cellulose synthase PCCESA1 protein from phytophthora capsici, and an encoding gene and application thereof. The PCCESA1 protein provided by the invention is the protein as shown in a sequence 2; the encoding gene of the protein is as shown in a sequence 1. An experiment proves that the protein PCCESA1 provided by the invention can catalyze substrate uridine diphosphate D glucose (UDPG) to generate cellobiose, and has significance on biosynthesis of main component cellulose of a cell wall of the phytophthora capsici; as an oomycetes cell wall plays an important role in maintaining oomycetes vital movement, including maintaining cell shapes, participating in host interaction and the like. Therefore, the cellulose synthase PCCESA1 protein provided by the invention provides technical support for the oomycetes cell wall, and provides a new molecular target for future bactericide research and development.

The invention relates to clone of phytophora capsic cellulose synthase related genes CesA3 and application of the clone in resistance detection of CAA bactericides, in particular to a method for detecting drug resistance molecules of phytophora capsic to the CAA bactericides and a special primer and belongs to the technical field of molecular biology. The primer consists of deoxyribonucleic acid (DNA) molecules represented by SEG ID NO:1 and SEG ID NO:2. The method for detecting drug resistance produced phytophora capsic point mutation to the CAA bactericides has the advantages of being high in sensitivity, simple, quick and stable in stality, can be used for quickly and sensitively detecting occurrence and development trends of resistance of the phytophora capsic in fields to the CAA bactericides and early warning resistance diseases, guiding and timely adjusting disease control strategies, and has great significance on effective control of development of the resistance diseases.

An antistaling method for fresh bamboo shoot includes such steps as preparing antistaling agent from aloe and water, coating it on the pretreated fresh bamboo shoot, drying in air, filming on its surface, microwave irradiating 5-20S and storage at 2-8 deg.C.

The invention relates to identification of nucleotide point mutation of a phytophthora capsici leonian-cellulose synthase-related gene CesA3 and a use of the phytophthora capsici leonian-cellulose synthase-related gene CesA3 in detection of resistance to pyrimorph as a fungicide, and discloses a molecular detection method and special primers for identifying phytophthora capsici leonian gene CesA3 3365-site nucleotide mutation-caused resistance to pyrimorph. The pair of special primers is composed of DNA molecules shown in the formulas of SEQ ID NO: 2 and SEQ ID NO: 3. The molecular diagnosis method for detecting phytophthora capsici leonian resistance to pyrimorph as a fungicide has high sensitivity, good stability and a short detection period, can be used for high-flux detection of generation and development of field phytophthora capsici leonian resistance to pyrimorph as a fungicide, realizes early warning of diseases with resistance and has an important meaning for timely and effective control of development of diseases with resistance.