A quantum digital signature (QDS) method using a labeled single photon source (HSPS) is divided into a preparation phase of the HSPS, a key distribution phase using a QDS scheme of the HSPS, and a signed message phase. Firstly, HSPS is obtained through down-conversion of spontaneous parameters, and after the marking effect is achieved, most of vacuum pulses are effectively eliminated. A key distribution protocol (KGP) is then performed on this basis such that participants in the signature protocol all obtain signed key strings. Finally, the transmission, verification and receiving (refusal) process of the signature information is carried out, and on the premise that the security of non-repudiation, unforgeability and transferability of the QDS protocol is met, whether the signature is valid or not is judged by comparing the mismatching rate between the key strings of the two parties. Compared with an existing QDS scheme using a weak coherent light source (WCS), the scheme has the advantages that the vacuum state proportion is reduced to a greater extent, the safe transmission distance is greatly increased, the method is efficient, feasible, simple and easy to operate, and a certainreference value is provided for the practicability of quantum cryptology.