The invention discloses a flap perforating branch artery locating method based on 3D printing reconstruction. The flap perforating branch artery locating method is characterized in that views and dataare transmitted to a processing device through DICOM images of human CT and MR, then aiming at the view and data, the processing device conducts the following processing steps that a, the views are compared and adjusted; b, the positions of selective points and determination points are firstly defined; c, a range domain is adjusted to determine the point-getting trend; d, point drawing tracing isconducted; e, a digitized 3D splitting model is manufactured; f, according to the model in the step e, 3D printing is conducted to obtain a physical model. The flap perforating branch artery locatingmethod has the advantages that the position of the flap perforating branch arteries can be accurately marked, at the same time, corresponding human organs are located and printed according to different parts, so that the surface of the 3D model has mark positions, corresponding to the skin, of the flap perforating branch arteries, the mark positions correspond to the human organs one to one, through model comparison, the positions, corresponding to the skin, of the human flap perforating branch arteries can be accurately judged, and the positions and shapes of hundreds of perforating branch arteries of all parts of the whole body can be accurately subjected to digitized virtual reconstruction and 3D physical printing.