The invention discloses a key needle tube type anti-theft anti-freezing sampling valve, and relates to an anti-theft anti-freezing sampling valve used for the mouth of an oil-water well in the oil extraction field. The key needle tube type anti-theft anti-freezing sampling valve comprises a valve body, a valve core, a driving mechanism and an anti-theft mechanism; the valve core is contained in an inner cavity of the valve body in form of capable of moving along the axial direction; one port of the inner cavity is used as an oil inlet and the other port is provided with a gland; a sampling channel interconnected with the inner cavity is arranged on the valve body; the valve core, under the drive of the driving mechanism, controls the opening and closing of the sampling channel by the reciprocating motion; the end of the valve core facing the oil inlet can extends out of the oil inlet. By adopting the key needle tube type anti-theft anti-freezing sampling valve, the blind section in front of the valve in the prior art is eliminated, and therefore, the crude oil is prevented from being accumulated and stored in the valve body, the stored oil is prevented from freezing, meanwhile, the exhausting is not required, in this way, the labor intensity of the operators in the filed is reduced, the environmental protection is helped, the sampling veracity is ensured, and further, the accidents of crude oil leakage and environment pollution caused by the fact that the sampling valve is opened by an irrelevant person are effectively prevented.