The invention discloses a mobile computed tomography (CT) scanner. The mobile CT scanner comprises a bottom plate platform, a machine frame, a rotary plate, an X-ray emitting source, and a detector and data acquisition system. The bottom plate platform is provided with a linear guide rail; and one end of the machine frame is provided with a guide rail sliding block which is slidably connected with the linear guide rail, and the other end of the machine frame is connected with the rotary plate. The rotary plate can rotate relative to the machine frame. The X-ray emitting source and the detector and data acquisition system are arranged on the rotary plate and are respectively positioned at two ends of the same diameter of the rotary plate. The X-ray emitting source comprises a grid controlled carbon nano tube-based field emission cathode X-ray tube. The invention also provides an operation method for the mobile CT scanner. The mobile CT scanner is simple in structure, low in radiation dosage, and high in imaging quality, and has two purposes.