The invention provides an automobile middle and rear floor assembly clamp. The automobile middle and rear floor assembly clamp comprises a platform, and the platform is provided with clamping mechanisms. Each clamping mechanism comprises a power source, a chuck and a supporting frame. The first clamping mechanism, the second clamping mechanism, the tenth clamping mechanism, the third clamping mechanism and the fourth clamping mechanism are sequentially arranged at one end of the platform, the eleventh clamping mechanism, the seventh clamping mechanism, the fourteenth clamping mechanism, the ninth clamping mechanism and the twelfth clamping mechanism are sequentially arranged at the other end of the platform, and the fifth clamping mechanism is arranged in the middle of the platform. According to the automobile middle and rear floor assembly clamp, because a middle and rear floor assembly of an automobile is characterized by being special in structure and irregular in part of the shape, the clamping mechanisms are arranged on the periphery and the middle of the platform respectively according to production requirements when the positions of all the clamping mechanisms are arranged, the middle and rear floor assembly of the automobile can be reasonably fixed, and the machining operation on the middle and rear floor assembly of the automobile is not affected on the premise that a good fixing effect is guaranteed.