33results about How to "Full length" patented technology

The invention provides a method for carrying out high-throughput sequencing on a TCR (T cell receptor) or a BCR (B cell receptor). The method is characterized by designing upstream primers according to gene features of a V region of the TCR or the BCR and designing downstream primers according to gene features of a C region or a J region of the TCR or the BCR and obtaining sequences of the of the TCR or the BCR in combination with the multiplex PCR (polymerase chain reaction) technology and high-throughput sequencing, thus analyzing the rearrangement information of the TCR or the BCR. Compared with 25-30 cycles of existing multiplex PCR, two cycles of the multiplex PCR technology provided by the invention can conduce to greatly reducing the sequencing errors caused by primer amplification preference. Besides, the invention also provides a method for correcting multiplex PCR (polymerase chain reaction) primer deviation by utilizing DNA (deoxyribonucleic acid) tag sequences, thus further reducing the sequencing errors caused by primer amplification preference and intrinsic sequencing errors of high-throughput sequencing.

The present invention discloses a refrigerator hinge which can pass through wire beam. It belongs to refrigerator design technical field. It provides a refrigerator hinge which can be hidden, can pass through wire beam, does not affect appearance and has long use life. It consists essentially of hinge seat used for fixing screw hole, cantilever connected with hinge seat and hinge axle of cantilever end, it is characterized by: it is equipped with a channel which can be used for laying wire beam from hinge seat, cantilever, axle seat to hinge axle, said channel consists of the access slit on the hinge seat, the threading groove connecting the access slit and the axle seat on the cantilever, the wire leading mouth on the lateral surface of hinge axle and the threading hole on the center of hinge axle sequentially. The distance between the wire beam leaded out the threading hole of the hinge axle and the display is longer, wire beam has full freedom degree, so the wreckage failure will not happen. The advantages are: compact structure, good concealment performance and versatility, long use life. It is mainly used for the hinge in the refrigerator.

The invention provides multiple PCR primers for constructing a leukemia minimal residual disease BCR library based on high-flux sequencing. The multiple PCR primers comprise upstream primers and downstream primers, wherein the upstream primers are an upstream primer group composed of nucleotide sequences which are 0-3 basic groups more or less than the nucleotide sequences shown in the SEQ ID NO: 1-SEQ ID NO: 13, and the downstream primers are composed of nucleotide sequences which are 0-3 basic groups more or less than the nucleotide sequences shown in the SEQ ID NO: 14-SEQ ID NO: 17. The provided multiple PCR primer group can efficiently construct the B lymphocyte receptor high-flux sequencing library of a leukemia patient, so that rich leukemia minimal residual disease BCR rearrangement information can be obtained.

The invention relates to a yarn splicing device for pneumatically joining yarns, especially elastic yarns, with a splicing body having a vacuum-loadable splicing channel which can be operated by means of a The covering element is closed and, moreover, a support is arranged in the region of the splicing channel, which supports the fixing of the yarn ends. According to the invention, it is provided that the supporting element consists of a spring wire (36) and a cover element (23), wherein the spring wire (36) is positioned in an opposite position in the spliced ​​body (19). In the groove (35) that moves angularly in the splicing channel (20), that is, the yarn ends (31, 32) to be spliced ​​are clamped between the covering element (23) and the spring wire ( 36) Between.

The present invention belongs to the field of tissue engineering and medical material technology, and is especially titanium net with fimbrin coating and its preparation process and application in reconstructing trachea default. The present invention prepares fimbrin-titanium net complex with medical titanium net as support material and fimbrin to form coating. Fimbrin has excellent biocompatibility and can promote the growth of fibroblast and mucosa cell, speed healing, reduce complications. Clinically, the present invention is used in repairing trachea default. The present invention is significant in the research of fimbrin in promoting repair of trachea mucosa, reconstructing trachea default and reducing complications during reconstructing trachea default.

The invention discloses a rubber composition and a processing method thereof, and application of the rubber composition to the processing of a white carbon black reinforced rubber product. The rubbercomposition comprises a rubber matrix and necessary components, wherein the rubber matrix comprises the following components: branched polyethylene with the content a being greater than 0 and smallerthan or equal to 100 parts; ethyene-propylene rubber and ethylene propylene terpolymer with the content b being greater than or equal to 0 and smaller than 100 parts; the necessary components comprisethe following components: 1 to 10 parts of a cross-linking agent, and 15 to 80 parts of white carbon black. The rubber composition has the benefits that the rubber composition has high mechanical strength, and can be used for producing high-voltage insulation sleeve size, high temperature-resistance conveying belts, waterproof roll, color rubber particles for surface layers of plastic runways, rubber plugs, rubber rolls, inner tubes, tire treads, side walls of tires, and inner rubber layers of air conditioner rubber tubes.

Provided is an immersion nozzle for continuous casting, in which the neck part of a cylindrical refractory object can be prevented from being damaged due to a pressing force or a stress by forming a metal case provided for the cylindrical refractory in a double structure configured from an outer part and an inner part. The immersion nozzle for continuous casting comprises an inner metal case (12)provided onto a part or the entire of the outer surface of the cylindrical refractory object (1), a tapered metal part (12a) so formed on the inner metal case (12) as to correspond to the tapered part (11a) of the cylindrical refractory object (1), and an outer metal case (13) provided outside of the inner metal case (12). At least parts of the metal cases (12, 13) are joined to each other.

The present invention relates to a combined-type wiring groove and combined-type table, pertaining to the field of furniture. The combined-type wiring groove comprises a wiring groove used for installing on upright columns. The wiring groove comprises at least two wiring single grooves. The neighboring wiring single grooves are combined and fixed with each other. The combined wiring single groovesare used for connecting different upright columns. According to the combined-type wiring groove, by adopting the structural design of the wiring single grooves, the usage requirements of the uprightcolumns with different distances are satisfied, and the unnecessary material waste is reduced at the present stage. The combined-type wiring groove is suitable for the using of small-specification orlarge-specification lifting tables, the stable combination requirements of different tables are realized, and the production efficiency in related industries is improved indirectly.

A test stand encompasses a dynamometer, which is supported to oscillate about a main axis, from which a lever arm extends perpendicularly. For calibrating a force-measuring device, which is coupled to the lever arm, provision is made for a pneumatic cylinder as reference force-generating device. The reference force can be measured by a load cell and can be applied to the lever arm via a yoke. The force-measuring device, which is to be calibrated, the pneumatic cylinder, the load cell, and the yoke are disposed on the same side of the dynamometer and act on the same lever arm.