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33results about How to "Full length" patented technology

Method for carrying out high-throughput sequencing on TCR (T cell receptor) or BCR (B cell receptor) and method for correcting multiplex PCR (polymerase chain reaction) primer deviation by utilizing tag sequences

The invention provides a method for carrying out high-throughput sequencing on a TCR (T cell receptor) or a BCR (B cell receptor). The method is characterized by designing upstream primers according to gene features of a V region of the TCR or the BCR and designing downstream primers according to gene features of a C region or a J region of the TCR or the BCR and obtaining sequences of the of the TCR or the BCR in combination with the multiplex PCR (polymerase chain reaction) technology and high-throughput sequencing, thus analyzing the rearrangement information of the TCR or the BCR. Compared with 25-30 cycles of existing multiplex PCR, two cycles of the multiplex PCR technology provided by the invention can conduce to greatly reducing the sequencing errors caused by primer amplification preference. Besides, the invention also provides a method for correcting multiplex PCR (polymerase chain reaction) primer deviation by utilizing DNA (deoxyribonucleic acid) tag sequences, thus further reducing the sequencing errors caused by primer amplification preference and intrinsic sequencing errors of high-throughput sequencing.
Owner:SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA +1

All-purpose refrigerator threading hinge

The present invention discloses a refrigerator hinge which can pass through wire beam. It belongs to refrigerator design technical field. It provides a refrigerator hinge which can be hidden, can pass through wire beam, does not affect appearance and has long use life. It consists essentially of hinge seat used for fixing screw hole, cantilever connected with hinge seat and hinge axle of cantilever end, it is characterized by: it is equipped with a channel which can be used for laying wire beam from hinge seat, cantilever, axle seat to hinge axle, said channel consists of the access slit on the hinge seat, the threading groove connecting the access slit and the axle seat on the cantilever, the wire leading mouth on the lateral surface of hinge axle and the threading hole on the center of hinge axle sequentially. The distance between the wire beam leaded out the threading hole of the hinge axle and the display is longer, wire beam has full freedom degree, so the wreckage failure will not happen. The advantages are: compact structure, good concealment performance and versatility, long use life. It is mainly used for the hinge in the refrigerator.
Owner:HAIER GRP CORP +1

Chopped glass fiber reinforced polypropylene composite material and preparation method and application thereof

The invention belongs to the technical field of polypropylene modification and discloses a chopped glass fiber reinforced polypropylene composite material and a preparation method and application thereof. The chopped glass fiber reinforced polypropylene composite material is prepared from the following ingredients in percentage by mass: 35-75% of polypropylene, 3-10% of grafted modified polypropylene, 20-60% of chopped glass fibers, 0.1-0.5% of antioxidant and 0.2-0.8% of lubricant. During the preparation of the chopped glass fiber reinforced polypropylene composite material disclosed by the invention, glass fiber dispersion zones of screws of a twin-screw extruder adopt large-lead and non-meshed screw elements, so that the damage and destruction to the glass fibers caused by chopping are reduced, and the reserved length of the glass fibers is guaranteed; and the obtained composite material has the advantages that the mechanical properties are excellent, the tensile strength reaches up to 132MPa, the bending strength reaches up to 187MPa, the bending modulus reaches up to 12,733MPa, and the notched impact strength reaches up to 51 KJ / m<2>.
Owner:WUHAN JINFA TECH CO LTD

Multiple PCR primers and method for constructing leukemia minimal residual disease BCR library based on high-flux sequencing

The invention provides multiple PCR primers for constructing a leukemia minimal residual disease BCR library based on high-flux sequencing. The multiple PCR primers comprise upstream primers and downstream primers, wherein the upstream primers are an upstream primer group composed of nucleotide sequences which are 0-3 basic groups more or less than the nucleotide sequences shown in the SEQ ID NO: 1-SEQ ID NO: 13, and the downstream primers are composed of nucleotide sequences which are 0-3 basic groups more or less than the nucleotide sequences shown in the SEQ ID NO: 14-SEQ ID NO: 17. The provided multiple PCR primer group can efficiently construct the B lymphocyte receptor high-flux sequencing library of a leukemia patient, so that rich leukemia minimal residual disease BCR rearrangement information can be obtained.
Owner:GENEMIND BIOSCIENCES CO LTD

Apparatus for splicing yarns pneumatically

The invention relates to a yarn splicing device for pneumatically joining yarns, especially elastic yarns, with a splicing body having a vacuum-loadable splicing channel which can be operated by means of a The covering element is closed and, moreover, a support is arranged in the region of the splicing channel, which supports the fixing of the yarn ends. According to the invention, it is provided that the supporting element consists of a spring wire (36) and a cover element (23), wherein the spring wire (36) is positioned in an opposite position in the spliced ​​body (19). In the groove (35) that moves angularly in the splicing channel (20), that is, the yarn ends (31, 32) to be spliced ​​are clamped between the covering element (23) and the spring wire ( 36) Between.
Owner:SAURER GMBH & CO KG

Titanium net with fimbrin and its prepn and application

The present invention belongs to the field of tissue engineering and medical material technology, and is especially titanium net with fimbrin coating and its preparation process and application in reconstructing trachea default. The present invention prepares fimbrin-titanium net complex with medical titanium net as support material and fimbrin to form coating. Fimbrin has excellent biocompatibility and can promote the growth of fibroblast and mucosa cell, speed healing, reduce complications. Clinically, the present invention is used in repairing trachea default. The present invention is significant in the research of fimbrin in promoting repair of trachea mucosa, reconstructing trachea default and reducing complications during reconstructing trachea default.
Owner:AFFILIATED HUSN HOSPITAL OF FUDAN UNIV +1

Three-dimensional rotary storage bin

The invention belongs to the field of rebar storage equipment and discloses a three-dimensional rotary storage bin. The three-dimensional rotary storage bin comprises a supporting frame. A rotary roller is rotationally arranged at the upper end of the supporting frame. At least one transportation chain wheel is fixedly arranged on the rotary roller. The transportation chain wheels are sleeved with transportation chains. A cylinder on the supporting frame is sleeved with the ends, far away from the transportation chain wheels, of the transportation chains. Multiple storage arms are arranged on each transportation chain. A drive device is arranged on the supporting frame and drives the rotary roller to rotate, and multiple guide grooves are arranged below the supporting frame in the length direction of the supporting frame, wherein the guide grooves and the transportation chains are arranged in a staggered manner. The transportation chains are driven by the rotating rotary roller to rotate, so that the storage arms can move along with the transportation chains so as to achieve feeding and discharging of rebars, the three-dimensional space is fully used, the occupied area is small, the space utilization rate is high, besides, the storage bin can rotate, fixed-point feeding is achieved, the discharging manner is diversified, up-claw type material taking as well as side-rotating type discharging are achieved, and therefore the feeding work of straight-strip rebars is more convenient to operate.
Owner:TJK MACHINERY TIANJIN

Rubber composition, processing method, application and production of application

The invention discloses a rubber composition and a processing method thereof, and application of the rubber composition to the processing of a white carbon black reinforced rubber product. The rubbercomposition comprises a rubber matrix and necessary components, wherein the rubber matrix comprises the following components: branched polyethylene with the content a being greater than 0 and smallerthan or equal to 100 parts; ethyene-propylene rubber and ethylene propylene terpolymer with the content b being greater than or equal to 0 and smaller than 100 parts; the necessary components comprisethe following components: 1 to 10 parts of a cross-linking agent, and 15 to 80 parts of white carbon black. The rubber composition has the benefits that the rubber composition has high mechanical strength, and can be used for producing high-voltage insulation sleeve size, high temperature-resistance conveying belts, waterproof roll, color rubber particles for surface layers of plastic runways, rubber plugs, rubber rolls, inner tubes, tire treads, side walls of tires, and inner rubber layers of air conditioner rubber tubes.
Owner:HANGZHOU XINGLU TECH CO LTD +1

Rubber composition, application to foam products and production method

The invention discloses rubber composition, a processing method thereof, an application of the composition to foam product production and a method. The rubber composition is prepared from a rubber matrix and essential components, wherein the rubber matrix contains a parts of branched polyethylene and b parts of EPM rubber and EPDM rubber, a is larger than 0 and smaller than or equal to 100 parts,and b is larger than or equal to 0 and smaller than 100 parts; the essential components comprise 0.5-10 parts of a crosslinking agent and 1.5-25 parts of a foaming agent. The rubber composition applies to processing of sponge sealing strips, foam materials with high foaming ratio, damping foam rubber boards, light-color high-strength foam boards, foam solid compound sealing strips as well as solidtires filled with foam elastomers. The rubber composition has the beneficial effects as follows: branched polyethylene not only contains short branch chains of methyl, ethyl, propyl, butyl, amyl andthe like, but also contains long branch chains with carbon number larger than 6, by replacing ethylene propylene rubber in the foam rubber composition partially or completely with branched polyethylene, melt strength of the rubber composition can be improved, foamability, especially pre-foamability of the composition is improved, and the obtained rubber products have lower compression set.
Owner:HANGZHOU XINGLU TECH CO LTD +1

Immersion nozzle for continuous casting

Provided is an immersion nozzle for continuous casting, in which the neck part of a cylindrical refractory object can be prevented from being damaged due to a pressing force or a stress by forming a metal case provided for the cylindrical refractory in a double structure configured from an outer part and an inner part. The immersion nozzle for continuous casting comprises an inner metal case (12)provided onto a part or the entire of the outer surface of the cylindrical refractory object (1), a tapered metal part (12a) so formed on the inner metal case (12) as to correspond to the tapered part (11a) of the cylindrical refractory object (1), and an outer metal case (13) provided outside of the inner metal case (12). At least parts of the metal cases (12, 13) are joined to each other.
Owner:SHINAGAWA REFRACTORIES CO LTD

Test Stand with an Apparatus for Calibrating a Force-Measuring Device

A test stand encompasses a dynamometer, which is supported to oscillate about a main axis, from which a lever arm extends perpendicularly. For calibrating a force-measuring device, which is coupled to the lever arm, provision is made for a pneumatic cylinder as reference force-generating device. The reference force can be measured by a load cell and can be applied to the lever arm via a yoke. The force-measuring device, which is to be calibrated, the pneumatic cylinder, the load cell, and the yoke are disposed on the same side of the dynamometer and act on the same lever arm.
Owner:HORIBA EURO

A three-dimensional rotary storage bin

The invention belongs to the field of rebar storage equipment and discloses a three-dimensional rotary storage bin. The three-dimensional rotary storage bin comprises a supporting frame. A rotary roller is rotationally arranged at the upper end of the supporting frame. At least one transportation chain wheel is fixedly arranged on the rotary roller. The transportation chain wheels are sleeved with transportation chains. A cylinder on the supporting frame is sleeved with the ends, far away from the transportation chain wheels, of the transportation chains. Multiple storage arms are arranged on each transportation chain. A drive device is arranged on the supporting frame and drives the rotary roller to rotate, and multiple guide grooves are arranged below the supporting frame in the length direction of the supporting frame, wherein the guide grooves and the transportation chains are arranged in a staggered manner. The transportation chains are driven by the rotating rotary roller to rotate, so that the storage arms can move along with the transportation chains so as to achieve feeding and discharging of rebars, the three-dimensional space is fully used, the occupied area is small, the space utilization rate is high, besides, the storage bin can rotate, fixed-point feeding is achieved, the discharging manner is diversified, up-claw type material taking as well as side-rotating type discharging are achieved, and therefore the feeding work of straight-strip rebars is more convenient to operate.
Owner:TJK MACHINERY TIANJIN

Repair method of flexible mineral insulated cable with metal outer sheath

The invention discloses a method for repairing a flexible mineral insulated cable with a metal outer sheath. The method is characterized by comprising the following steps of: measuring and positioninga breakdown point by using a cable fault positioning instrument, respectively measuring linear distances from the breakdown point to two ends of a faulty cable, indicating that the faulty cable can be repaired if the linear distance from the breakdown point to one end of the faulty cable is within 100 meters, and defining the end as a first end; installing the faulty cable on the pay-off rack, pulling the first end head, pulling out the faulty cable, and pulling the faulty cable to a position where the breakdown point is exposed; cutting off the metal outer sheath, and pulling the metal outersheath forwards to expose the breakdown point; replacing the damaged or blackened insulating mica tape to restore the insulating property of the insulating mica tape, and winding the wrapping tape outside the insulating mica tape again; and welding the metal outer sheath together in a butt joint manner to obtain a qualified cable with repaired insulation performance. The method has the advantagesthat the method can be used for repairing the quality problem of the flexible mineral insulated cable, and scrap and inventory are effectively reduced.
Owner:宁波东方电缆股份有限公司

Assembled blade guniting stirring drill bit and using method thereof

The invention relates to an assembled blade guniting stirring drill bit and a using method thereof. The drill bit is composed of a hexagonal male joint, a drill rod body, an upper guniting opening, a clamping plate, a bolt, assembled blades, an auxiliary clamping plate, a nut, a core pipe, a lower guniting opening, a sealing gasket, guniting blade assemblies, an air spraying opening and a drill tip, wherein the hexagonal male joint is welded to the top end of the drill rod body, the guniting blade assemblies are arranged at the upper end and the lower end of the drill rod body, the multiple assembled blades are arranged at intervals in the period, and the drill rod body is provided with the upper guniting opening, the lower guniting opening and the air spraying opening. The problems that the guniting amount of the edge area of a large-diameter stirring pile is insufficient, and stirring is not uniform are effectively solved, the integrity of a pile body of the stirring pile is remarkably improved, the pile forming quality is good, and safety and reliability are achieved.
Owner:城地建设集团有限公司 +2
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