40results about How to "Great amplification" patented technology

Disclosed are compositions and a method for amplification of nucleic acid sequences of interest. The disclosed method generally involves replication of a target sequence such that, during replication, the replicated strands are displaced from the target sequence by strand displacement replication of another replicated strand. In one form of the disclosed method, the target sample is not subjected to denaturing conditions. It was discovered that the target nucleic acids, genomic DNA, for example, need not be denatured for efficient multiple displacement amplification. The primers used can be hexamer primers. The primers can also each contain at least one modified nucleotide such that the primers are nuclease resistant. The primers can also each contain at least one modified nucleotide such that the melting temperature of the primer is altered relative to a primer of the same sequence without the modified nucleotide(s). The DNA polymerase can be φ29 DNA polymerase.

A multi-hop wireless, for example cellular, communications system is provided comprising a source equipment which may be one of a base station or an end user terminal for transmitting signals towards a destination equipment which may be the other of a base station or an end user terminal via at least one relay equipment. The relay equipment receives a plurality of signals transmitted from one or more source equipments of the system and from this plurality of signals selects a signal to relay. In this way the decision about which relays are included in which communication paths in the system is distributed to the relay equipments of the system, thus reducing the signalling overhead as compared with link state protocols which are typically used for routing in such multi-hop systems.

A method for investigating specimens, wherein a spectral splitting of the radiation coming from the specimen is carried out for specimen points or point distributions, for the operation of a laser scanning microscope or a fluorescence screening arrangement or a flow cylinder comprising the steps of generating a λ-stack so that the spectral distribution is measured by individual detection channels and storing the signals so as to be correlated to the detection signals with at least one of the spatial coordinates x, y and z and / or so as to be correlated to the measurement time t.

Disclosed are compositions and a method for amplification of nucleic acid sequences of interest. The disclosed method generally involves replication of a target sequence such that, during replication, the replicated strands are displaced from the target sequence by strand displacement replication of another replicated strand. In one form of the disclosed method, the target sample is not subjected to denaturing conditions. It was discovered that the target nucleic acids, genomic DNA, for example, need not be denatured for efficient multiple displacement amplification. The primers used can be hexamer primers. The primers can also each contain at least one modified nucleotide such that the primers are nuclease resistant. The primers can also each contain at least one modified nucleotide such that the melting temperature of the primer is altered relative to a primer of the same sequence without the modified nucleotide(s). The DNA polymerase can be φ29 DNA polymerase.

Avalanche photodiodes (APDs) and single photon avalanche detectors (SPADs) are provided with a lateral multiplication region that provides improved amplification through increased impact ionization.

A multi-hop wireless, for example cellular, communications system is provided comprising a source equipment which may be one of a base station or an end user terminal for transmitting signals towards a destination equipment which may be the other of a base station or an end user terminal via at least one relay equipment. The relay equipment receives a plurality of signals transmitted from one or more source equipments of the system and from this plurality of signals selects a signal to relay. In this way the decision about which relays are included in which communication paths in the system is distributed to the relay equipments of the system, thus reducing the signalling overhead as compared with link state protocols which are typically used for routing in such multi-hop systems.

Arrangement and method for the optical detection of light radiation which is excited and / or backscattered in a specimen, wherein the illumination of the specimen and / or the detection of the specimen light is carried out by at least two objectives arranged on different sides of the specimen. The specimen illumination is focused in or in the vicinity of a pupil plane of the beam path between the specimen plane and the detection plane at least on one axis and an element for the spatial separation of the illumination light from the detection light are provided in this plane.

Arrangement for the optical detection of light radiation which is excited and / or backscattered in a specimen in a microscope, wherein the specimen illumination is focused in and / or in the vicinity of a pupil plane of the beam path between the specimen plane and detection plane and apparatus is provided in this plane for spatially separating the illumination light from the detection light, wherein fluorescent light and / or luminescent light and / or phosphorescent light and / or diffusely scattered illumination light coming from the specimen is detected, and the apparatus for spatial separation comprises at least a reflecting first portion and at least a transmitting second portion, wherein the reflecting portion serves to couple in the illumination light and the transmitting portion serves to pass the detection light in the detection direction or the transmitting portion serves to couple in the illumination light and the reflecting portion serves to couple out the detection light.

Method and / or arrangement for identifying fluorescing, luminescing and / or absorbing substances on and / or in sample carriers, particularly with high sample throughput in sample screening and / or in diagnostics, preferably in the analysis of samples in microtiter plates (MTP), wherein a spectral splitting of the sample light is carried out and detection is carried out in a plurality of detection channels, and at least one summation and / or combination of the signals of the individual channels is carried out for at least a portion of the detection channels. In an advantageous manner, at least one standard sample (STD) and / or at least one blank sample (BLK) are / is arranged on the sample carrier in addition to the substances (PRB) to be examined, and a spectrum of at least one standard sample (STD) is recorded in a first step.

Methods are provided for determining, in a single polymerase chain reaction (PCR) reaction, the quantity, quality, and gender of origin of DNA in a sample, and whether or not the sample contains PCR amplification inhibitors. The methods involve carrying out a single PCR multiplex reaction utilizing primer sets specific for amplifying the human amelogenin locus, an X- and / or Y-chromosome specific gene that is shorter than the amelogenin gene, and a heterologous, non-human reporter gene.

In the field of knock detection in a combustion engine, a method and a device detects and controls the knock by adapting the gain of an amplifier which amplifies the knock sensor signal. The gain of the amplifier is adapted based on analysis of a set of samples from the knock sensor so that the amplification of the knock sensor signal across the entire measuring window is optimum and the complete ADC evaluation range can be utilized better.

A laser gain module comprises a spoiled hexagonal shaped slab for receiving a laser beam. The laser beam enters the slab on one face and is reflected internally at one or more faces. The laser beam propagates through the slab and may be amplified with each reflection. The laser gain module also comprises two opposing non-parallel sides that are elongated in comparison to the remaining four sides thus creating the spoiled hexagon geometry. The module may also include two or four laser diode arrays positioned so as to constitute a side pumping arrangement to provide additional energy to the laser gain module. The additional energy provided to the laser gain module may result in greater amplification of the laser beam as it propagates through the laser gain module.

Methods of identifying specific target molecules for design of anti-angiogenic and vascular targeting approaches are disclosed. Transcriptional profiles of tumor endothelial cells were compared with that of normal resting endothelial cells, normal but angiogenically activated placental endothelial cells, and cultured endothelial cells. Although the majority of transcripts were classified as general angiogenesis markers, 17 genes were identified that show specific overexpression in tumor endothelium. Antibody targeting of four cell-surface expressed or secreted products (vimentin, CD59, HMGB1 and IGFBP7) inhibited angiogenesis in vitro and in vivo. Finally, targeting endothelial vimentin in a mouse tumor model significantly inhibited tumor growth and reduced microvessel density. The results demonstrate the utility of the identification and subsequent targeting of specific tumor endothelial markers for anticancer therapy.

The invention relates to an injection valve (1), which is used in particular as an injector for fuel injection systems or exhaust gas aftertreatment systems, comprising a shock wave actuator (4), a valve closing body (8) that interacts with a valve seat surface (7) to form a sealing seat (9), and a shock wave amplification channel (22). The shock wave amplification channel (22) is used to conduct shock waves (27) generated by the shock wave actuator (4) to the sealing seat (9) and to amplify said shock waves (27).

The invention concerns an audio signal processing unit including a receiving unit adapted to receive at least a first audio signal and a pilot tone signal, a first audio signal amplifier adapted to obtain at least a second audio signal from an audio signal source by way of an audio signal input, and a pilot tone signal unit adapted to obtain the received pilot tone signal. The pilot tone signal unit is adapted to output a control signal in a first state if the pilot tone signal is received, and to output the control signal in a second state if the pilot tone signal is not received. The first audio signal amplifier is adapted to process the at least second audio signal in dependence on the state of the control signal.

The invention relates to an optical amplifying structure capable of amplifying at least one light wave S, comprising in a substrate, for each wave to be amplified, an amplifying assembly composed of: a first micro-waveguide (7) capable of receiving the light wave S to be amplified, a second micro-waveguide (9) capable of receiving a pumping wave L, a multiplexing device (11) associated with the first and second micro-waveguides, and capable of providing a coupled light wave composed of the wave S and the wave L, an amplifying device (13) connected to an output of the multiplexing device and capable of amplifying the light wave S by at least partial absorption of the pumping wave L, the amplifying device being capable of providing at one output the amplified light wave S. a third micro-waveguide (15) connected to the output of the amplifying device and capable of carrying the amplified light wave S, and a demultiplexing device (19) associated with the third micro-waveguide and capable of demultiplexing the pumping wave L from the amplified wave S, and of providing on a fourth micro-waveguide (17) an amplified light wave S, purged of the pumping wave. The structure of the invention is applied to all fields necessitating an amplification of a light wave and in particular in the field of optical telecommunications by optical fibres.

A first signal path having an amplifier and a second signal path having an amplifier with adjustable gain factor are provided. A signal applied to the first and second signal paths is amplified and demodulated on the first signal path. Concurrently, the signal is amplified on the second signal path with a gain factor, and a power of the signal amplified by the second signal path is determined and used for determining the gain factor. A signal conditioning circuit has first and second signal paths and a first and a second operating state. In the first operating state, the first signal path is arranged for amplification for a demodulation, and the second signal path is arranged for amplification for determination of a power of the signal present. In the second operating state, one of the two signal paths is inactive and the other is arranged for demodulating the signal present.

The invention relates to a ground arrangement of a device using wireless data transfer. In order to improve the electric properties of the device and to minimize the SAR value of the device, at least one electrically conductive additional ground lead (73-75) is formed in the device in addition to the actual ground lead (72). In between the ground lead and the additional ground lead, a galvanic coupling is automatically established, when a predetermined switching criterion is fulfilled, and the aforementioned galvanic coupling is automatically released, when a predetermined release criterion is fulfilled. This enables one to change the ground configuration of an antenna such that it is as suitable as possible for the transmission and reception circumstances each time existing.

A micro angular rate-sensing device is provided. A vibrator, having a plurality of proof masses connected to a ring, is arranged in a first base. Flexible supporting members connect to the vibrator to suspend the vibrator in the first base. Electrodes drive the vibrator to oscillate and control the oscillation mode of the vibrator in driving mode. Planar electrodes are arranged relative to the proof masses on a second base. The motion of the proof masses relative to the planar electrodes is sensed through the capacitance between the proof masses and the planar electrodes. The resonant structure with greater mass of the device generates greater Coriolis force and increases the sensing area. Thus, the intensity of sensed signals and noise-signal ratio are greatly increased. Furthermore, the device does not rely much on high aspect ratio manufacturing technology. Thus the manufacturing cost is reduced and the yield rate is increased.

The present invention provides replicating [100K−] adenovirus vectors that have an impairment in 100K activity. In particular preferred embodiments, the impairment is the result of a deletion in the 100K coding region of the adenovirus vector genome. It is further preferred that the adenovirus produces the E1 gene products. In an alternate embodiment, the adenovirus produces the E1a gene products, but has an impairment in the E1b coding region, such that replication of the virus is limited to p53− cells. Also described are methods of making and administering the inventive adenovirus vectors to a cell or to a subject. Further provided is use of the inventive [100K−] Ad vectors as a helper virus for the production of vector stocks of adeno-associated virus.

Method and arrangement for changing the spectral composition and / or intensity of illumination light and / or specimen light in an adjustable manner, wherein a spatial separation into radiation components of different polarization is carried out with a first polarizing device, a spectral, spatial splitting of at least one radiation component is carried out with first dispersion device, the polarization state of at least one part of the spectrally spatially split radiation component is changed, and a spatial separation and / or combination of radiation components of different polarization are / is carried out by a second polarizing device, wherein a spatial combination of radiation components which are changed and not changed with respect to their polarization state is advantageously carried out by a second dispersion device.

The invention relates to a device for frequency conversion, comprising exactly one multi-band mixer (3) with means for transferring a signal (2) that is applied to the signal input of said multi-band mixer (3) from said signal's source frequency band to one out of a plurality of target frequency sub-bands at a time, yielding a transferred signal (4) at the signal output of said multi-band mixer (3), wherein said plurality of target frequency sub-bands defines at least a first and a second target frequency band, and a bank of switchable Variable Gain Amplifiers (VGAs) (5-1, 5-2) for amplifying said transferred signal (4), wherein for each of said target frequency bands, one switchable VGA (5-1, 5-2) is provided that is adapted to said target frequency band and is connected to the signal output of said multi-band mixer (3). The invention also relates to a method for frequency conversion.

The invention provides highly sensitive, specific and efficient quantitative real-time PCR compositions, methods and assay kits to detect at least one IFN subtype and / or IFN subtype allotypic variants. Primer / probe sets complementary to the coding sequence of an IFN subtype of interest avoid spurious detection of degraded mRNA and enhances the correlation between the IFN subtype that is measured by the assays of the invention and the protein that is actually expressed. The invention also provides methods for designing primers and methods of using the compositions and assay kits. The compositions, kits, and methods of the invention may be used, for example, to monitor vaccine efficacy, autoimmune disease, chronic infections, or tumor therapy.

The current invention is a novel approach termed “parallel screening” which allows simultaneously screening of a population for insertions in all genes cloned from that or a closely related organism. In order to test this approach, the flowering plant Petunia hybrida was used as a model system. Petunia hybrida line W137 contains a high copy number of the endogenous transposable element dTph1 and has been previously presented as a genetic tool. A 3D library of the plant genomic DNA of 1000 Petunia hybrida W137 plants was generated. The 3D library consists of 30 pools of DNA from 100 plants each. These were used to generate 30 pools of insertion flanking sequences by nested iPCR using a set of transposon-specific primers or by Transposon Display PCR. Insertions into a gene were detected by hybridizing the amplified insertion flanking sequences fixed to a filter with a gene-specific probe, an approach termed simple screening for insertion elements. Alternatively, the amplified insertion element flanking sequences were labeled and used as a probe to hybridize a filter displaying multiple gene targets, an approach termed parallel screening for insertion elements, which allows the simultaneous screening for insertions in all genes of an organism, appearing in a population of insertion mutants.