The invention discloses sterilization slow release cream for pets and a preparation method of the cream. The cream is prepared from components in percentage by mass as follows: 0.1%-3% of a radix astragalus extract, 0.1%-3% of an aloe extract, 0.1%-2% of a radix ginseng extract, 0.1%-2% of a radix ophiopogonis extract, 0.1%-2% of a fructus schisandrae extract, 5%-15% of milk powder, 2%-10% of chicken liver powder, 2%-10% of fish meal, 0.5%-3% of lecithin, 5%-15% of white sugar, 1%-10% of maltose, 0.5%-3% of fructooligosaccharide, 0.5%-3% of sodium carboxymethylcellulose, 10%-18% of bovine hydrolysis extract, 10%-18% of fish oil, 0.1%-0.35% of sodium benzoate, 0.001% of vitamin A, 0.02% of vitamin B1, 0.02% of vitamin B2, 0.5% of vitamin C, 0.2% of vitamin E, 0.5% of zinc sulfate and the balance of water. Therefore, the components with the sterilization slow release effect can be absorbed effectively, negative emotions of pets after sterilization operations can be relieved, wound healing can be accelerated, and immunity can be enhanced.