The invention discloses a preparation method of cefotetan disodium, which belongs to the technical field of medicine and comprises the following steps: A. Adding methanol into the reactor, stirring and controlling the temperature, then adding cefotetan acid, and then adding a salt-forming agent, stirring until dissolved to obtain a mixed solution; B. Control the temperature of the reactor, add a small amount of organic solvent until the system is slightly cloudy, and grow crystals once; C. After the first crystal growth is completed, add an organic solvent, carry out secondary crystal growth, and filter to obtain solid crystals of cefotetan disodium; D. The solid crystal of cefotetan disodium is washed, atomized and dried, and vacuum-dried to obtain the finished product of cefotetan disodium. The cefotetan disodium prepared by the invention has low impurity content, good fluidity, uniform particle size distribution, stable quality, easy subpackage of subsequent powder injection preparation products, and improved drug safety; the preparation method of the invention also has the advantages of simple operation , The advantages of energy saving and environmental protection.