The invention relates to a biological sample detection vehicle and a vehicular detection system thereof. At present, a mobile lab achieving biosafety level-3 protection level is developed at home and abroad, which is called 'P3 vehicle' commonly, conventional home and abroad P3 vehicle is formed by grouping more than 2 box-type vehicles, and has large size and complicated operation. The invention aims at providing a miniature biological sample detection vehicle which has excellent maneuvering characteristic and biosafety protection function, adapts to epidemic situations or public health incidents and consists of a cockpit (1), a front cabin (9), a rear cabin (22) and an atmosphere sampler (19), wherein the front cabin is an equipment cabin, and the rear cabin is an experimental cabin. The biological sample detection vehicle integrates biological sampling, air pre-alarming and quick detection into a whole, has good maneuvering property and matched functions, has the function of carrying out various biological sampling and pathogenic microorganism detection in the field of epidemic situation in remote rural areas or urban roadways, and can be used for executing a 'bio-control' quick detection task in contingency medical rescue with high efficiency.