The present invention discloses an interference spray test device for an opposed piston engine. The device comprises a constant volume bomb system, an air intake and exhaust system, a fuel oil jettingsystem, a high-speed photographing system and a control and acquisition system; the constant volume bomb system is used for simulating the internal condition of a combustion chamber when opposed pistons are located at inner dead points so as to realize a fuel oil jetting, collision and atomization experiment; the air intake and exhaust system is used for providing corresponding jetting back pressure for the interior of the constant volume bomb of the constant volume bomb system and discharging oil mist exhaust gases in the process of the experiment; the fuel oil jetting system is used for establishing oil pressure meeting the requirements of the experiment and realizing fuel oil jetting; and the high-speed photographing system is used for capturing and recording spray structures in a spray field; and the control and acquisition system controls a spray direction, oil jetting pressure and a photographing process, realizes the photographing control of fuel oil jetting and atomization andobtains the process image of spray development, and completes the acquisition of test data. With the test device of the invention adopted, on the one hand, jetting back pressure in a cylinder can beeffectively simulated, and on the other hand, flexible adjustment of interference spray at different angles can be realized.