The invention discloses a pedicle positioning and guiding device for percutaneous vertebroplasty, which comprises a sleeve provided with a puncture hole, and also comprises a spinous process fixing rod, wherein the spinous process fixing rod is vertically connected with a graduated scale, the graduated scale is slidably provided with a sliding rod, the sliding rod is respectively perpendicular tothe graduated scale and the spinous process fixing rod, the sliding rod is rotatably provided with a base, and a rotating shaft of the base is respectively perpendicular to the sliding rod and the spinous process fixing rod; the sleeve is connected with the base, the base is fixedly provided with an angle disc, and the sleeve and the angle disc are respectively perpendicular to the rotating shaftof the base. The invention also discloses an operation method thereof, wherein the spinous process fixing rod is fixed at the highest position of the spinous process and is perpendicular to the longitudinal axis of the vertebral body, the sliding rod and the sleeve are adjusted so that the sliding rod reaches a displacement of L, and the sleeve rotates an angle alpha and is aligned with a first positioning point. Compared with the prior art, the device has accurate positioning, can reduce the fluoroscopy times of the X-ray machine and shorten operation time and bleeding amount in an operation.The device is used for simulation teaching, shortens the learning curve during vertebral body forming, and is more suitable for practical application.