4 results about "Proline Hydroxylase" patented technology

Provided is a method for preparing a recombinant human collagen. The method comprises: constructing a genetically engineered bacterium which expresses a prolyl hydroxylase and a recombinant human collagen; culturing the genetically engineered bacterium and performing digestion treatment by means of pepsin to obtain a recombinant human collagen type I or type III. The prolyl hydroxylase comprises P4Ha2, P4Hb and vP4H, and by means of controlling the ratio of the copy numbers of the genes thereof, the recombinant human collagen and a natural human collagen are consistent in hydroxylation modification. Also provided is a large-scale production method for the recombinant human collagen. The prepared recombinant human collagen has completely the same sequence composition and length and substantially the same hydroxyproline content as the natural human collagen, and also has a stable full-length triple helix structure and biological activity without immunogenicity and potential risk of viruses.

This invention relates to the construction of a human placental-derived proline hydroxylase α subunit mutant and its expression vector. Through analysis and screening of the natural hydroxylase subunit, a mutant protein and its construct that are efficiently expressed in Ecoli BL21(DE3) with significantly improved hydroxylation efficiency were obtained.

The application discloses a recombinant collagen III+X, a preparation method and application thereof, and belongs to the technical field of bio-chemical industry. The recombinant collagen III+X provided by the application has an amino acid sequence shown as SEQ ID NO:1, is composed of a human collagen III 387-467 amino acid fragment repeated twice, and is connected with a human collagen X 521-680 amino acid fragment at a C terminal. Experiments prove that the recombinant collagen III+X can form a stable triple helix structure by co-expression with a proline hydroxylase, has great advantages in cell migration and safety, and can be used as a raw material for preparation of wound dressings and skin care products.