The invention discloses a synthesis method of a multifrequency synchronous signal source, comprising the following steps of, determining an amount N of main frequencies in a multifrequency synchronous signal to be synthesized, thereby determining that the signal is subdivided into 2N equal parts within a period T0, wherein N should be an odd number; finding out vectors of first N symmetric odd functions SAL (20, t), SAL (21, t), ......, SAL (2N-1, t) in a Walsh function system within a period, wherein each vector contains 2N elements; summing N vectors to obtain a sum vector g (N, t); carrying out normalization treatment on the sum vector g (N, t) with a sign function sgn () pair, thereby obtaining vectors f (N, t) of multifrequency synchronous signals of +1 and -1 within the period T0; and repeating the f (N, t) with a fundamental frequency period T0 to obtain a periodic multifrequency synchronous signal. The multifrequency synchronous signal source synthesized through the method hasvarious very ideal time domain and frequency domain characteristics and realizes multifrequency synchronous measurement of an impedance spectroscopy.