The invention discloses a turbine blade vibration test tool and a turbine blade clamping method, the vibration test tool comprises a main body, the main body is internally provided with an opening alignment chute inner cavity and a force application step hole, the included angle of the opening alignment chute inner cavity is the same as the included angle of blade tenon teeth, and the opening alignment chute inner cavity is used for placing a clamping block; the clamping block is provided with a clamping mortise and a large arc transition hollow elastic section, one end of the clamping block is provided with a threaded hole communicated with the hollow elastic section, and the threaded hole is used for installing a stress application screw. The other end of the connecting rod is connectedwith the nut; the stop block tightly abuts against the tenon bottom of the blade under the action of the adjusting bolt. The stress application screw penetrates through a stress application stepped hole in the main body and extends into the threaded hole of the clamping block, the stop block penetrates through the main body and the clamping block, and an adjusting bolt used for adjusting the position of the stop block is installed on the main body. According to the structure, the blade tenon is fixedly held through the combined action of pressing and jacking, so that the attachment area of thetenon tooth surface of the blade and a tool mortise is large, the contact is uniform, the fixedly holding rigidity is high, and the consistency is good.