The invention discloses a method for immunizing animals by using a needleless injector, which comprises the following steps: S1, preparing an antigen mixed solution, the antigen mixed solution comprises DNA expression plasmid, CPG-ODN adjuvant and normal saline, and the DNA expression plasmid is used as a specific antigen; s2, performing needleless injection immunization on an immune animal, and injecting the prepared antigen mixed solution into the immune animal body by using a needleless injector, so that an immune reaction occurs in the immune animal body, and an antibody aiming at a specific antigen is generated; and S3, repeatedly performing needle-free injection immunization, and after 2-6 times of needle-free injection immunization, detecting the titer of the antibody aiming at the specific antigen in the immune animal serum. According to the immunization method provided by the invention, the immune response of animals is greatly improved, more opportunities are provided for research and development of monoclonal antibody drugs, the administration process is simplified by a needle-free injection immunization mode, the pain and complications of experimental animals are reduced, and the occupational safety of experimental operators can also be improved.