The embodiment of the utility model discloses a closed pipeline control device for realizing calibration, split charging and exhaust, which comprises a pipeline, a bracket, a
control unit, a
peristaltic pump, an
air filter, a bubble sensor group and a
pinch valve group, the bubble sensor group and the
pinch valve group are respectively connected on the bracket, the
peristaltic pump is connected on the bracket, and the
control unit is connected on the bracket. One end of the pipeline is connected with the
stock solution bag, the other end of the pipeline passes through the
peristaltic pump, the other end of the pipeline is further connected with a plurality of
branch pipes, the
branch pipes are connected with sub-packaging bags, a first groove body is arranged in the bubble sensor group, and the
branch pipes are clamped in the first groove body; a second groove body is arranged in the
pinch valve group, and the branch
pipe is clamped in the second groove body; the pipeline is further connected with an
air filter, and the
control unit is connected with the peristaltic pump, the bubble sensor set and the pinch valve set. By implementing the device disclosed by the embodiment of the utility model, a sealed pipeline
system can be adopted, so that the device has lower background environment requirements and
pollution risks, higher subpackaging precision, more thorough bubble removal and higher safety and reliability during
cryopreservation.