The invention discloses a large-section tunnel excavation and support construction method for crossing a soil-rock boundary stratum. The method comprises the following steps of first, upper middle step excavation and initial support construction, specifically, carrying out upper step excavation and initial support construction on a construction tunnel by a plurality of excavation sections from rear to front, and when any excavation section is subjected to upper step excavation and initial support construction, the process comprises the following steps of measuring pay-off, drilling and blasting construction, excavation and initial support of an upper cave, and excavation and initial support of a middle cave; second, lower step excavation and initial support construction; and third, carrying out secondary lining construction. The method is reasonable in design, simple and convenient to construct and good in use effect, the upper blasting area in the upper step and the middle blasting area on the middle step are separately subjected to blasting zone division, the arrangement position of each shot hole in each blasting zone is defined, the collapse of a tunnel face or the collapse ofa vault caused by large vibration generated by blasting when one-time loading of the large section is excessive can be effectively prevented, the safety and reliability of the tunnel excavation process are ensured, and the stability of the tunnel structure is ensured.