The invention provides a fluorescent VPCR identification method for a
pinellia ternata adulterated counterfeit product, and belongs to the technical field of
drug detection. According to the identification method established by the invention, the extraction time of the
DNA of the
pinellia ternate medicinal material can be remarkably shortened, one or more of four traditional Chinese medicinal materials such as
pinellia ternate, brooklet
anemone root, rhizoma typhonii flagelliformis and rhizoma arisaematis and decoction pieces can be identified at the same time, the identification duration is controlled within 1 hour, and meanwhile, the method has excellent specificity, sensitivity, accuracy and applicability and can be widely applied to the field of clinical application. The method can identify 5% of mixed products adulterated in
Pinellia ternate, and can provide a new means for
quality monitoring of
Pinellia ternate medicinal materials and decoction pieces thereof, thereby ensuring lawful rights and interests of consumers and maintaining the
normal order of the traditional Chinese medicinal material market.