The invention relates to a laboratory instrument storage fixation device and an operation method. The device comprises a fixed frame, a mounting frame, regulating sleeves, regulating rods, rotating sleeves, sliding plates, protective sleeves, mounting sleeves, and sliding blocks. The lower part of the fixed frame is connected to a fixed plate, the upper part of the fixed plate is connected to the mounting frame, the mounting frame slides on the fixed plate, two sides of the mounting frame fit the fixed frame, the inside of the fixed frame is connected to the mounting frame, the inner side of the mounting frame is connected to a laboratory instrument, both sides of the mounting frame are equipped with regulating sleeve slots, the regulating sleeve slots fit the regulating sleeves, the regulating sleeves are connected to the regulating sleeve slots through fastening screws, the insides of the regulating sleeves fit the regulating rods, the regulating rods are connected to the regulating sleeves, the regulating sleeves and the regulating rods are engaged with each other, the regulating rods have rotating bulges, the inside of the mounting frame is connected to the sliding plates, and the sliding plates slide in the mounting frame.