The embodiment of the invention relates to the technical field of communication, and discloses a packet loss rate calculation method, a server and a computer readable storage medium. In the invention,the packet loss rate calculation method is applied to a first agent end based on a forward error correction (FEC) protocol, and comprises the following steps: determining a maximum serial number, a minimum serial number and the number of received messages in FDP format messages received in a sampling period, wherein the message in the FDP format is sent by the second agent end and comprises a serial number field, and the serial number field comprises serial number information of the message; calculating a first packet loss rate according to the maximum serial number, the minimum serial number, the number of the messages and a preset constraint relationship among the maximum serial number, the minimum serial number, the number of the messages and the packet loss rate, wherein the first packet loss rate is the packet loss rate of the second agent end. In the embodiment of the invention, the packet loss rate can be calculated without sending the detection message, the calculation processof the packet loss rate is simplified, the detection message is prevented from occupying the bandwidth, and the flow consumption is reduced.