The present disclosure provides improved viscosity measuring assemblies, and related methods of use. More particularly, the present disclosure provides advantageous measuring assemblies configured to measure the viscosity of samples / fluids (e.g., opaque or transparent liquids) using microchannels. The present disclosure provides for a viscosity measuring assembly (e.g., hand-held electronic measuring assembly) that is configured to measure the viscosity of samples / fluids (e.g., opaque or transparent Newtonian and non-Newtonian liquids, including blood, etc.), in a short period of time (e.g., within a couple of minutes) utilizing only about a droplet of fluid. The viscosity measuring assembly can include a substrate having a microchannel, a light source (e.g., a collimated low coherence light source), and a sensor (e.g., photodiode). The micro-channeled substrate may be disposable, and some of the other components may be reusable since they are substantially not directly exposed to the sample during operation of the viscosity measuring assembly.