The invention discloses a natural rubber composition material as well as a preparation method and application thereof. A raw material composition of the natural rubber composition material is preparedfrom the following components according to the parts by weight: 85-92 parts of natural rubber, 8-15 parts of butadiene rubber, 4-10 parts of activator A, 0.5-2 parts of activator B, 2-6 parts of anti-aging agent, 2-4 parts of protective wax, 1-3 parts of tackifier, 10-25 parts of carbon black, 3-8 parts of white carbon black, 1-1.4 parts of vulcanizing agent, 0.1-0.4 parts of accelerant A, and 1.2-2.5 parts of accelerant B. The natural rubber composition material prepared from the invention is low in damping coefficient, and excellent in mechanical property, and capable of improving the fatigue radiating feature of the material, relieving the fracture and damage of a rubber chain caused by the fatigue rapid heating, improving the durability life, improving the high temperature resistancefeature of the vulcanized rubber, and reducing the high temperature creep deformations of the vulcanized rubber. A prepared automobile engine suspension damping material NVH has the good control effect, and the service life of a road test reaches the standard.